Section 1. Identification
QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
CHEMTREC®: 1-800-424-9300
SAFETY DATA SHEET
Product name
In case of emergency
:
:
Supplier/Manufacturer
:
Agilent Technologies, Inc.
5301 Stevens Creek Blvd
Santa Clara, CA 95051, USA
800-227-9770
QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Conforms to US OSHA Hazard Communication 29CFR1910.1200
Part no. (chemical kit)
:
200522
QuikSolution
200516-51
PfuUltra HF DNA Polymerase
200524-51
10X Reaction Buffer
200518-58
Dpn I
200518-52
Control Primer 1 (34-mer)
200518-53
Control Primer 2 (34-mer)
200518-54
pWS4.5 Control Template
200518-55
QuikChange XL dNTP Mix
200516-52
XL10-Gold Ultracompetent cells
200315-41
XL10-Gold 2-Mercaptoethanol
200314-43
pUC 18 DNA Control Plasmid
200231-42
:
Part no.
Validation date
:
12/16/2022
1.2 Relevant identified uses of the substance or mixture and uses advised against
1.1 Product identifier
1.3 Details of the supplier of the safety data sheet
1.4 Emergency telephone number
Identified uses
Analytical reagent.
QuikSolution
0.5 ml
PfuUltra HF DNA Polymerase
0.032 ml (80 U 2.5 U/µl)
10X Reaction Buffer
0.5 ml
Dpn I
0.03 ml (10 U/µl 300 U)
Control Primer 1 (34-mer)
0.0075 ml (750 ng 100 ng/ µl)
Control Primer 2 (34-mer)
0.0075 ml (750 ng 100 ng/ µl)
pWS4.5 Control Template
0.01 ml (50 ng 5 ng/ µl)
QuikChange XL dNTP Mix
0.03 ml
XL10-Gold Ultracompetent cells
1.35 ml (10 x 0.135 ml)
XL10-Gold 2-Mercaptoethanol
2 x 0.05 ml
pUC 18 DNA Control Plasmid
0.01 ml (0.1 ng / µl)
:
Section 2. Hazards identification
OSHA/HCS status
:
QuikSolution
This material is considered hazardous by the OSHA
Hazard Communication Standard (29 CFR 1910.1200).
PfuUltra HF DNA
Polymerase
This material is considered hazardous by the OSHA
Hazard Communication Standard (29 CFR 1910.1200).
10X Reaction Buffer
This material is considered hazardous by the OSHA
Hazard Communication Standard (29 CFR 1910.1200).
Dpn I
This material is considered hazardous by the OSHA
Hazard Communication Standard (29 CFR 1910.1200).
Control Primer 1 (34-mer)
While this material is not considered hazardous by the
OSHA Hazard Communication Standard (29 CFR
2.1 Classification of the substance or mixture
1/52
Date of issue :
12/16/2022
QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 2. Hazards identification
Classification of the substance or mixture
1910.1200), this SDS contains valuable information
critical to the safe handling and proper use of the product.
This SDS should be retained and available for employees
and other users of this product.
Control Primer 2 (34-mer)
While this material is not considered hazardous by the
OSHA Hazard Communication Standard (29 CFR
1910.1200), this SDS contains valuable information
critical to the safe handling and proper use of the product.
This SDS should be retained and available for employees
and other users of this product.
pWS4.5 Control Template
While this material is not considered hazardous by the
OSHA Hazard Communication Standard (29 CFR
1910.1200), this SDS contains valuable information
critical to the safe handling and proper use of the product.
This SDS should be retained and available for employees
and other users of this product.
QuikChange XL dNTP Mix
While this material is not considered hazardous by the
OSHA Hazard Communication Standard (29 CFR
1910.1200), this SDS contains valuable information
critical to the safe handling and proper use of the product.
This SDS should be retained and available for employees
and other users of this product.
XL10-Gold Ultracompetent
cells
This material is considered hazardous by the OSHA
Hazard Communication Standard (29 CFR 1910.1200).
XL10-Gold
2-Mercaptoethanol
This material is considered hazardous by the OSHA
Hazard Communication Standard (29 CFR 1910.1200).
pUC 18 DNA Control
Plasmid
While this material is not considered hazardous by the
OSHA Hazard Communication Standard (29 CFR
1910.1200), this SDS contains valuable information
critical to the safe handling and proper use of the product.
This SDS should be retained and available for employees
and other users of this product.
QuikSolution
H227
FLAMMABLE LIQUIDS - Category 4
H320
EYE IRRITATION - Category 2B
PfuUltra HF DNA Polymerase
H320
EYE IRRITATION - Category 2B
10X Reaction Buffer
H319
EYE IRRITATION - Category 2A
H412
AQUATIC HAZARD (LONG-TERM) - Category 3
Dpn I
H320
EYE IRRITATION - Category 2B
XL10-Gold Ultracompetent cells
H320
EYE IRRITATION - Category 2B
XL10-Gold 2-Mercaptoethanol
H318
SERIOUS EYE DAMAGE - Category 1
H317
SKIN SENSITIZATION - Category 1
H361
TOXIC TO REPRODUCTION - Category 2
H373
SPECIFIC TARGET ORGAN TOXICITY (REPEATED EXPOSURE) - Category 2
H412
AQUATIC HAZARD (LONG-TERM) - Category 3
2/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 2. Hazards identification
Signal word
:
QuikSolution
Warning
PfuUltra HF DNA Polymerase
Warning
10X Reaction Buffer
Warning
Dpn I
Warning
Control Primer 1 (34-mer)
No signal word.
Control Primer 2 (34-mer)
No signal word.
pWS4.5 Control Template
No signal word.
QuikChange XL dNTP Mix
No signal word.
XL10-Gold Ultracompetent cells
Warning
XL10-Gold 2-Mercaptoethanol
Danger
pUC 18 DNA Control Plasmid
No signal word.
Hazard statements
:
QuikSolution
H227 - Combustible liquid.
H320 - Causes eye irritation.
PfuUltra HF DNA Polymerase
H320 - Causes eye irritation.
10X Reaction Buffer
H319 - Causes serious eye irritation.
H412 - Harmful to aquatic life with long lasting
effects.
Dpn I
H320 - Causes eye irritation.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
H320 - Causes eye irritation.
XL10-Gold 2-Mercaptoethanol
H317 - May cause an allergic skin reaction.
H318 - Causes serious eye damage.
H361 - Suspected of damaging fertility or the
unborn child.
H373 - May cause damage to organs through
prolonged or repeated exposure.
H412 - Harmful to aquatic life with long lasting
effects.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
Hazard pictograms
:
10X Reaction Buffer
XL10-Gold 2-Mercaptoethanol
Precautionary statements
Prevention
:
QuikSolution
P210 - Keep away from flames and hot surfaces.
No smoking.
PfuUltra HF DNA Polymerase
Not applicable.
10X Reaction Buffer
P280 - Wear eye or face protection.
P273 - Avoid release to the environment.
Dpn I
Not applicable.
Control Primer 1 (34-mer)
Not applicable.
2.2 GHS label elements
QuikChange XL dNTP Mix
Percentage of the mixture consisting of ingredient
(s) of unknown hazards to the aquatic environment:
5.7%
XL10-Gold Ultracompetent cells
Percentage of the mixture consisting of ingredient
(s) of unknown hazards to the aquatic environment:
5%
3/52
Date of issue :
12/16/2022
QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 2. Hazards identification
Control Primer 2 (34-mer)
Not applicable.
pWS4.5 Control Template
Not applicable.
QuikChange XL dNTP Mix
Not applicable.
XL10-Gold Ultracompetent cells
Not applicable.
XL10-Gold 2-Mercaptoethanol
P201 - Obtain special instructions before use.
P280 - Wear protective gloves, protective clothing
and eye or face protection.
P273 - Avoid release to the environment.
P260 - Do not breathe vapor.
pUC 18 DNA Control Plasmid
Not applicable.
Response
:
QuikSolution
P305 + P351 + P338 - IF IN EYES: Rinse
cautiously with water for several minutes. Remove
contact lenses, if present and easy to do. Continue
rinsing.
P337 + P313 - If eye irritation persists: Get medical
advice or attention.
PfuUltra HF DNA Polymerase
P305 + P351 + P338 - IF IN EYES: Rinse
cautiously with water for several minutes. Remove
contact lenses, if present and easy to do. Continue
rinsing.
P337 + P313 - If eye irritation persists: Get medical
advice or attention.
10X Reaction Buffer
P305 + P351 + P338 - IF IN EYES: Rinse
cautiously with water for several minutes. Remove
contact lenses, if present and easy to do. Continue
rinsing.
P337 + P313 - If eye irritation persists: Get medical
advice or attention.
Dpn I
P305 + P351 + P338 - IF IN EYES: Rinse
cautiously with water for several minutes. Remove
contact lenses, if present and easy to do. Continue
rinsing.
P337 + P313 - If eye irritation persists: Get medical
advice or attention.
Control Primer 1 (34-mer)
Not applicable.
Control Primer 2 (34-mer)
Not applicable.
pWS4.5 Control Template
Not applicable.
QuikChange XL dNTP Mix
Not applicable.
XL10-Gold Ultracompetent cells
P305 + P351 + P338 - IF IN EYES: Rinse
cautiously with water for several minutes. Remove
contact lenses, if present and easy to do. Continue
rinsing.
P337 + P313 - If eye irritation persists: Get medical
advice or attention.
XL10-Gold 2-Mercaptoethanol
P308 + P313 - IF exposed or concerned: Get
medical advice or attention.
P363 - Wash contaminated clothing before reuse.
P302 + P352 - IF ON SKIN: Wash with plenty of
water.
P333 + P313 - If skin irritation or rash occurs: Get
medical advice or attention.
P305 + P351 + P338, P310 - IF IN EYES: Rinse
cautiously with water for several minutes. Remove
contact lenses, if present and easy to do. Continue
rinsing. Immediately call a POISON CENTER or
doctor.
pUC 18 DNA Control Plasmid
Not applicable.
4/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 2. Hazards identification
Storage
:
QuikSolution
P403 + P235 - Store in a well-ventilated place.
Keep cool.
PfuUltra HF DNA Polymerase
Not applicable.
10X Reaction Buffer
Not applicable.
Dpn I
Not applicable.
Control Primer 1 (34-mer)
Not applicable.
Control Primer 2 (34-mer)
Not applicable.
pWS4.5 Control Template
Not applicable.
QuikChange XL dNTP Mix
Not applicable.
XL10-Gold Ultracompetent cells
Not applicable.
XL10-Gold 2-Mercaptoethanol
Not applicable.
pUC 18 DNA Control Plasmid
Not applicable.
Disposal
:
QuikSolution
P501 - Dispose of contents and container in
accordance with all local, regional, national and
international regulations.
PfuUltra HF DNA Polymerase
Not applicable.
10X Reaction Buffer
P501 - Dispose of contents and container in
accordance with all local, regional, national and
international regulations.
Dpn I
Not applicable.
Control Primer 1 (34-mer)
Not applicable.
Control Primer 2 (34-mer)
Not applicable.
pWS4.5 Control Template
Not applicable.
QuikChange XL dNTP Mix
Not applicable.
XL10-Gold Ultracompetent cells
Not applicable.
XL10-Gold 2-Mercaptoethanol
P501 - Dispose of contents and container in
accordance with all local, regional, national and
international regulations.
pUC 18 DNA Control Plasmid
Not applicable.
Supplemental label
elements
:
QuikSolution
None known.
PfuUltra HF DNA Polymerase
None known.
10X Reaction Buffer
None known.
Dpn I
None known.
Control Primer 1 (34-mer)
None known.
Control Primer 2 (34-mer)
None known.
pWS4.5 Control Template
None known.
QuikChange XL dNTP Mix
None known.
XL10-Gold Ultracompetent cells
None known.
XL10-Gold 2-Mercaptoethanol
None known.
pUC 18 DNA Control Plasmid
None known.
Hazards not otherwise
classified
:
QuikSolution
None known.
PfuUltra HF DNA Polymerase
None known.
10X Reaction Buffer
None known.
Dpn I
None known.
Control Primer 1 (34-mer)
None known.
Control Primer 2 (34-mer)
None known.
pWS4.5 Control Template
None known.
QuikChange XL dNTP Mix
None known.
XL10-Gold Ultracompetent cells
None known.
XL10-Gold 2-Mercaptoethanol
None known.
pUC 18 DNA Control Plasmid
None known.
2.3 Other hazards
5/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 3. Composition/information on ingredients
Substance/mixture
:
QuikSolution
Substance
PfuUltra HF DNA Polymerase
Mixture
10X Reaction Buffer
Mixture
Dpn I
Mixture
Control Primer 1 (34-mer)
Mixture
Control Primer 2 (34-mer)
Mixture
pWS4.5 Control Template
Mixture
QuikChange XL dNTP Mix
Mixture
XL10-Gold Ultracompetent cells
Mixture
XL10-Gold 2-Mercaptoethanol
Mixture
pUC 18 DNA Control Plasmid
Mixture
Ingredient name
CAS number
%
There are no additional ingredients present which, within the current knowledge of the supplier and in the
concentrations applicable, are classified and hence require reporting in this section.
Occupational exposure limits, if available, are listed in Section 8.
Any concentration shown as a range is to protect confidentiality or is due to batch variation.
QuikSolution
Dimethyl sulfoxide
100
67-68-5
PfuUltra HF DNA Polymerase
Glycerol
≥50 - ≤75
56-81-5
Poly(oxy-1,2-ethanediyl), .alpha.-[(1,1,3,3-tetramethylbutyl)phenyl]-.omega.-
hydroxy-
<0.25
9036-19-5
10X Reaction Buffer
Ammonium sulphate
≤3
7783-20-2
Polyoxyethylene octyl phenyl ether
<2.5
9002-93-1
Dpn I
Glycerol
≥50 - ≤75
56-81-5
XL10-Gold Ultracompetent cells
Glycerol
≥10 - ≤25
56-81-5
Dimethyl sulfoxide
≤10
67-68-5
Potassium chloride
≤3
7447-40-7
XL10-Gold 2-Mercaptoethanol
2-Mercaptoethanol
≤5
60-24-2
6/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
QuikSolution
Immediately flush eyes with plenty of water,
occasionally lifting the upper and lower eyelids.
Check for and remove any contact lenses.
Continue to rinse for at least 10 minutes. If
irritation persists, get medical attention.
PfuUltra HF DNA Polymerase
Immediately flush eyes with plenty of water,
occasionally lifting the upper and lower eyelids.
Check for and remove any contact lenses.
Continue to rinse for at least 10 minutes. If
irritation persists, get medical attention.
10X Reaction Buffer
Immediately flush eyes with plenty of water,
occasionally lifting the upper and lower eyelids.
Check for and remove any contact lenses.
Continue to rinse for at least 10 minutes. Get
medical attention.
Dpn I
Immediately flush eyes with plenty of water,
occasionally lifting the upper and lower eyelids.
Check for and remove any contact lenses.
Continue to rinse for at least 10 minutes. If
irritation persists, get medical attention.
Control Primer 1 (34-mer)
Immediately flush eyes with plenty of water,
occasionally lifting the upper and lower eyelids.
Check for and remove any contact lenses. Get
medical attention if irritation occurs.
Control Primer 2 (34-mer)
Immediately flush eyes with plenty of water,
occasionally lifting the upper and lower eyelids.
Check for and remove any contact lenses. Get
medical attention if irritation occurs.
pWS4.5 Control Template
Immediately flush eyes with plenty of water,
occasionally lifting the upper and lower eyelids.
Check for and remove any contact lenses. Get
medical attention if irritation occurs.
QuikChange XL dNTP Mix
Immediately flush eyes with plenty of water,
occasionally lifting the upper and lower eyelids.
Check for and remove any contact lenses. Get
medical attention if irritation occurs.
XL10-Gold Ultracompetent cells
Immediately flush eyes with plenty of water,
occasionally lifting the upper and lower eyelids.
Check for and remove any contact lenses.
Continue to rinse for at least 10 minutes. If
irritation persists, get medical attention.
XL10-Gold 2-Mercaptoethanol
Get medical attention immediately. Call a poison
center or physician. Immediately flush eyes with
plenty of water, occasionally lifting the upper and
lower eyelids. Check for and remove any contact
lenses. Continue to rinse for at least 10 minutes.
Chemical burns must be treated promptly by a
physician.
pUC 18 DNA Control Plasmid
Immediately flush eyes with plenty of water,
occasionally lifting the upper and lower eyelids.
Check for and remove any contact lenses. Get
medical attention if irritation occurs.
Section 4. First aid measures
Eye contact
:
4.1 Description of necessary first aid measures
7/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 4. First aid measures
QuikSolution
Remove victim to fresh air and keep at rest in a
position comfortable for breathing. If not
breathing, if breathing is irregular or if respiratory
arrest occurs, provide artificial respiration or
oxygen by trained personnel. It may be
dangerous to the person providing aid to give
mouth-to-mouth resuscitation. Get medical
attention if adverse health effects persist or are
severe. If unconscious, place in recovery position
and get medical attention immediately. Maintain
an open airway. Loosen tight clothing such as a
collar, tie, belt or waistband.
PfuUltra HF DNA Polymerase
Remove victim to fresh air and keep at rest in a
position comfortable for breathing. If not
breathing, if breathing is irregular or if respiratory
arrest occurs, provide artificial respiration or
oxygen by trained personnel. It may be
dangerous to the person providing aid to give
mouth-to-mouth resuscitation. Get medical
attention if adverse health effects persist or are
severe. If unconscious, place in recovery position
and get medical attention immediately. Maintain
an open airway. Loosen tight clothing such as a
collar, tie, belt or waistband.
10X Reaction Buffer
Remove victim to fresh air and keep at rest in a
position comfortable for breathing. If not
breathing, if breathing is irregular or if respiratory
arrest occurs, provide artificial respiration or
oxygen by trained personnel. It may be
dangerous to the person providing aid to give
mouth-to-mouth resuscitation. Get medical
attention if adverse health effects persist or are
severe. If unconscious, place in recovery position
and get medical attention immediately. Maintain
an open airway. Loosen tight clothing such as a
collar, tie, belt or waistband. In case of inhalation
of decomposition products in a fire, symptoms
may be delayed. The exposed person may need
to be kept under medical surveillance for 48
hours.
Dpn I
Remove victim to fresh air and keep at rest in a
position comfortable for breathing. If not
breathing, if breathing is irregular or if respiratory
arrest occurs, provide artificial respiration or
oxygen by trained personnel. It may be
dangerous to the person providing aid to give
mouth-to-mouth resuscitation. Get medical
attention if adverse health effects persist or are
severe. If unconscious, place in recovery position
and get medical attention immediately. Maintain
an open airway. Loosen tight clothing such as a
collar, tie, belt or waistband.
Control Primer 1 (34-mer)
Remove victim to fresh air and keep at rest in a
position comfortable for breathing. Get medical
attention if symptoms occur.
Control Primer 2 (34-mer)
Remove victim to fresh air and keep at rest in a
position comfortable for breathing. Get medical
attention if symptoms occur.
pWS4.5 Control Template
Remove victim to fresh air and keep at rest in a
position comfortable for breathing. Get medical
Inhalation
:
8/52
Date of issue :
12/16/2022
QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 4. First aid measures
QuikSolution
Flush contaminated skin with plenty of water.
Remove contaminated clothing and shoes. Get
medical attention if symptoms occur. Wash
clothing before reuse. Clean shoes thoroughly
before reuse.
PfuUltra HF DNA Polymerase
Flush contaminated skin with plenty of water.
Remove contaminated clothing and shoes. Get
medical attention if symptoms occur. Wash
clothing before reuse. Clean shoes thoroughly
before reuse.
10X Reaction Buffer
Flush contaminated skin with plenty of water.
Remove contaminated clothing and shoes. Get
medical attention if symptoms occur. Wash
clothing before reuse. Clean shoes thoroughly
before reuse.
Dpn I
Flush contaminated skin with plenty of water.
Remove contaminated clothing and shoes. Get
medical attention if symptoms occur. Wash
clothing before reuse. Clean shoes thoroughly
before reuse.
Control Primer 1 (34-mer)
Flush contaminated skin with plenty of water.
Remove contaminated clothing and shoes. Get
attention if symptoms occur.
QuikChange XL dNTP Mix
Remove victim to fresh air and keep at rest in a
position comfortable for breathing. Get medical
attention if symptoms occur. In case of inhalation
of decomposition products in a fire, symptoms
may be delayed. The exposed person may need
to be kept under medical surveillance for 48
hours.
XL10-Gold Ultracompetent cells
Remove victim to fresh air and keep at rest in a
position comfortable for breathing. If not
breathing, if breathing is irregular or if respiratory
arrest occurs, provide artificial respiration or
oxygen by trained personnel. It may be
dangerous to the person providing aid to give
mouth-to-mouth resuscitation. Get medical
attention if adverse health effects persist or are
severe. If unconscious, place in recovery position
and get medical attention immediately. Maintain
an open airway. Loosen tight clothing such as a
collar, tie, belt or waistband.
XL10-Gold 2-Mercaptoethanol
Get medical attention immediately. Call a poison
center or physician. Remove victim to fresh air
and keep at rest in a position comfortable for
breathing. If it is suspected that fumes are still
present, the rescuer should wear an appropriate
mask or self-contained breathing apparatus. If
not breathing, if breathing is irregular or if
respiratory arrest occurs, provide artificial
respiration or oxygen by trained personnel. It
may be dangerous to the person providing aid to
give mouth-to-mouth resuscitation. If
unconscious, place in recovery position and get
medical attention immediately. Maintain an open
airway. Loosen tight clothing such as a collar, tie,
belt or waistband.
pUC 18 DNA Control Plasmid
Remove victim to fresh air and keep at rest in a
position comfortable for breathing. Get medical
attention if symptoms occur.
Skin contact
:
9/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 4. First aid measures
QuikSolution
Wash out mouth with water. Remove dentures if
any. If material has been swallowed and the
exposed person is conscious, give small quantities
of water to drink. Stop if the exposed person feels
sick as vomiting may be dangerous. Do not induce
vomiting unless directed to do so by medical
personnel. If vomiting occurs, the head should be
kept low so that vomit does not enter the lungs.
Get medical attention if adverse health effects
persist or are severe. Never give anything by
mouth to an unconscious person. If unconscious,
place in recovery position and get medical attention
immediately. Maintain an open airway. Loosen
tight clothing such as a collar, tie, belt or waistband.
PfuUltra HF DNA Polymerase
Wash out mouth with water. Remove dentures if
any. If material has been swallowed and the
exposed person is conscious, give small quantities
of water to drink. Stop if the exposed person feels
sick as vomiting may be dangerous. Do not induce
vomiting unless directed to do so by medical
personnel. If vomiting occurs, the head should be
kept low so that vomit does not enter the lungs.
Get medical attention if adverse health effects
persist or are severe. Never give anything by
mouth to an unconscious person. If unconscious,
place in recovery position and get medical attention
immediately. Maintain an open airway. Loosen
tight clothing such as a collar, tie, belt or waistband.
10X Reaction Buffer
Wash out mouth with water. Remove dentures if
any. If material has been swallowed and the
exposed person is conscious, give small quantities
of water to drink. Stop if the exposed person feels
medical attention if symptoms occur.
Control Primer 2 (34-mer)
Flush contaminated skin with plenty of water.
Remove contaminated clothing and shoes. Get
medical attention if symptoms occur.
pWS4.5 Control Template
Flush contaminated skin with plenty of water.
Remove contaminated clothing and shoes. Get
medical attention if symptoms occur.
QuikChange XL dNTP Mix
Flush contaminated skin with plenty of water.
Remove contaminated clothing and shoes. Get
medical attention if symptoms occur.
XL10-Gold Ultracompetent cells
Flush contaminated skin with plenty of water.
Remove contaminated clothing and shoes. Get
medical attention if symptoms occur. Wash
clothing before reuse. Clean shoes thoroughly
before reuse.
XL10-Gold 2-Mercaptoethanol
Get medical attention immediately. Call a poison
center or physician. Wash with plenty of soap and
water. Remove contaminated clothing and shoes.
Wash contaminated clothing thoroughly with water
before removing it, or wear gloves. Continue to
rinse for at least 10 minutes. Chemical burns must
be treated promptly by a physician. In the event of
any complaints or symptoms, avoid further
exposure. Wash clothing before reuse. Clean
shoes thoroughly before reuse.
pUC 18 DNA Control Plasmid
Flush contaminated skin with plenty of water.
Remove contaminated clothing and shoes. Get
medical attention if symptoms occur.
Ingestion
:
10/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 4. First aid measures
sick as vomiting may be dangerous. Do not induce
vomiting unless directed to do so by medical
personnel. If vomiting occurs, the head should be
kept low so that vomit does not enter the lungs.
Get medical attention if adverse health effects
persist or are severe. Never give anything by
mouth to an unconscious person. If unconscious,
place in recovery position and get medical attention
immediately. Maintain an open airway. Loosen
tight clothing such as a collar, tie, belt or waistband.
Dpn I
Wash out mouth with water. Remove dentures if
any. If material has been swallowed and the
exposed person is conscious, give small quantities
of water to drink. Stop if the exposed person feels
sick as vomiting may be dangerous. Do not induce
vomiting unless directed to do so by medical
personnel. If vomiting occurs, the head should be
kept low so that vomit does not enter the lungs.
Get medical attention if adverse health effects
persist or are severe. Never give anything by
mouth to an unconscious person. If unconscious,
place in recovery position and get medical attention
immediately. Maintain an open airway. Loosen
tight clothing such as a collar, tie, belt or waistband.
Control Primer 1 (34-mer)
Wash out mouth with water. If material has been
swallowed and the exposed person is conscious,
give small quantities of water to drink. Do not
induce vomiting unless directed to do so by medical
personnel. Get medical attention if symptoms
occur.
Control Primer 2 (34-mer)
Wash out mouth with water. If material has been
swallowed and the exposed person is conscious,
give small quantities of water to drink. Do not
induce vomiting unless directed to do so by medical
personnel. Get medical attention if symptoms
occur.
pWS4.5 Control Template
Wash out mouth with water. If material has been
swallowed and the exposed person is conscious,
give small quantities of water to drink. Do not
induce vomiting unless directed to do so by medical
personnel. Get medical attention if symptoms
occur.
QuikChange XL dNTP Mix
Wash out mouth with water. If material has been
swallowed and the exposed person is conscious,
give small quantities of water to drink. Do not
induce vomiting unless directed to do so by medical
personnel. Get medical attention if symptoms
occur.
XL10-Gold Ultracompetent cells
Wash out mouth with water. Remove dentures if
any. If material has been swallowed and the
exposed person is conscious, give small quantities
of water to drink. Stop if the exposed person feels
sick as vomiting may be dangerous. Do not induce
vomiting unless directed to do so by medical
personnel. If vomiting occurs, the head should be
kept low so that vomit does not enter the lungs.
Get medical attention if adverse health effects
persist or are severe. Never give anything by
mouth to an unconscious person. If unconscious,
place in recovery position and get medical attention
11/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 4. First aid measures
immediately. Maintain an open airway. Loosen
tight clothing such as a collar, tie, belt or waistband.
XL10-Gold 2-Mercaptoethanol
Get medical attention immediately. Call a poison
center or physician. Wash out mouth with water.
Remove dentures if any. If material has been
swallowed and the exposed person is conscious,
give small quantities of water to drink. Stop if the
exposed person feels sick as vomiting may be
dangerous. Do not induce vomiting unless directed
to do so by medical personnel. If vomiting occurs,
the head should be kept low so that vomit does not
enter the lungs. Chemical burns must be treated
promptly by a physician. Never give anything by
mouth to an unconscious person. If unconscious,
place in recovery position and get medical attention
immediately. Maintain an open airway. Loosen
tight clothing such as a collar, tie, belt or waistband.
pUC 18 DNA Control Plasmid
Wash out mouth with water. If material has been
swallowed and the exposed person is conscious,
give small quantities of water to drink. Do not
induce vomiting unless directed to do so by medical
personnel. Get medical attention if symptoms
occur.
4.2 Most important symptoms/effects, acute and delayed
Inhalation
:
QuikSolution
No known significant effects or critical hazards.
PfuUltra HF DNA Polymerase
No known significant effects or critical hazards.
10X Reaction Buffer
No known significant effects or critical hazards.
Dpn I
No known significant effects or critical hazards.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
No known significant effects or critical hazards.
XL10-Gold 2-Mercaptoethanol
No known significant effects or critical hazards.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
Skin contact
:
QuikSolution
No known significant effects or critical hazards.
PfuUltra HF DNA Polymerase
No known significant effects or critical hazards.
10X Reaction Buffer
No known significant effects or critical hazards.
Dpn I
No known significant effects or critical hazards.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
No known significant effects or critical hazards.
XL10-Gold 2-Mercaptoethanol
May cause an allergic skin reaction.
QuikSolution
Causes eye irritation.
PfuUltra HF DNA Polymerase
Causes eye irritation.
10X Reaction Buffer
Causes serious eye irritation.
Dpn I
Causes eye irritation.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
Causes eye irritation.
XL10-Gold 2-Mercaptoethanol
Causes serious eye damage.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
:
Eye contact
Potential acute health effects
12/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 4. First aid measures
QuikSolution
No known significant effects or critical hazards.
PfuUltra HF DNA Polymerase
No known significant effects or critical hazards.
10X Reaction Buffer
No known significant effects or critical hazards.
Dpn I
No known significant effects or critical hazards.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
No known significant effects or critical hazards.
XL10-Gold 2-Mercaptoethanol
No known significant effects or critical hazards.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
:
Ingestion
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
Over-exposure signs/symptoms
Inhalation
QuikSolution
No specific data.
PfuUltra HF DNA Polymerase
No specific data.
10X Reaction Buffer
No specific data.
Dpn I
No specific data.
Control Primer 1 (34-mer)
No specific data.
Control Primer 2 (34-mer)
No specific data.
pWS4.5 Control Template
No specific data.
QuikChange XL dNTP Mix
No specific data.
XL10-Gold Ultracompetent cells
No specific data.
XL10-Gold 2-Mercaptoethanol
Adverse symptoms may include the following:
reduced fetal weight
increase in fetal deaths
skeletal malformations
pUC 18 DNA Control Plasmid
No specific data.
:
Eye contact
:
QuikSolution
Adverse symptoms may include the following:
irritation
watering
redness
PfuUltra HF DNA Polymerase
Adverse symptoms may include the following:
irritation
watering
redness
10X Reaction Buffer
Adverse symptoms may include the following:
pain or irritation
watering
redness
Dpn I
Adverse symptoms may include the following:
irritation
watering
redness
Control Primer 1 (34-mer)
No specific data.
Control Primer 2 (34-mer)
No specific data.
pWS4.5 Control Template
No specific data.
QuikChange XL dNTP Mix
No specific data.
XL10-Gold Ultracompetent cells
Adverse symptoms may include the following:
irritation
watering
redness
XL10-Gold 2-Mercaptoethanol
Adverse symptoms may include the following:
pain
watering
redness
pUC 18 DNA Control Plasmid
No specific data.
13/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 4. First aid measures
Notes to physician
:
QuikSolution
Treat symptomatically. Contact poison treatment
specialist immediately if large quantities have been
ingested or inhaled.
PfuUltra HF DNA Polymerase
Treat symptomatically. Contact poison treatment
specialist immediately if large quantities have been
ingested or inhaled.
10X Reaction Buffer
In case of inhalation of decomposition products in a
fire, symptoms may be delayed. The exposed
person may need to be kept under medical
surveillance for 48 hours.
Dpn I
Treat symptomatically. Contact poison treatment
specialist immediately if large quantities have been
ingested or inhaled.
Control Primer 1 (34-mer)
Treat symptomatically. Contact poison treatment
specialist immediately if large quantities have been
ingested or inhaled.
Control Primer 2 (34-mer)
Treat symptomatically. Contact poison treatment
specialist immediately if large quantities have been
ingested or inhaled.
pWS4.5 Control Template
Treat symptomatically. Contact poison treatment
specialist immediately if large quantities have been
ingested or inhaled.
QuikChange XL dNTP Mix
In case of inhalation of decomposition products in a
fire, symptoms may be delayed. The exposed
person may need to be kept under medical
Skin contact
Ingestion
QuikSolution
No specific data.
PfuUltra HF DNA Polymerase
No specific data.
10X Reaction Buffer
No specific data.
Dpn I
No specific data.
Control Primer 1 (34-mer)
No specific data.
Control Primer 2 (34-mer)
No specific data.
pWS4.5 Control Template
No specific data.
QuikChange XL dNTP Mix
No specific data.
XL10-Gold Ultracompetent cells
No specific data.
XL10-Gold 2-Mercaptoethanol
Adverse symptoms may include the following:
stomach pains
reduced fetal weight
increase in fetal deaths
skeletal malformations
pUC 18 DNA Control Plasmid
No specific data.
QuikSolution
No specific data.
PfuUltra HF DNA Polymerase
No specific data.
10X Reaction Buffer
No specific data.
Dpn I
No specific data.
Control Primer 1 (34-mer)
No specific data.
Control Primer 2 (34-mer)
No specific data.
pWS4.5 Control Template
No specific data.
QuikChange XL dNTP Mix
No specific data.
XL10-Gold Ultracompetent cells
No specific data.
XL10-Gold 2-Mercaptoethanol
Adverse symptoms may include the following:
pain or irritation
redness
blistering may occur
reduced fetal weight
increase in fetal deaths
skeletal malformations
pUC 18 DNA Control Plasmid
No specific data.
:
:
4.3 Indication of immediate medical attention and special treatment needed, if necessary
14/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 4. First aid measures
Protection of first-aiders
:
QuikSolution
No action shall be taken involving any personal risk
or without suitable training. It may be dangerous to
the person providing aid to give mouth-to-mouth
resuscitation.
PfuUltra HF DNA Polymerase
No action shall be taken involving any personal risk
or without suitable training. It may be dangerous to
the person providing aid to give mouth-to-mouth
resuscitation.
10X Reaction Buffer
No action shall be taken involving any personal risk
or without suitable training. It may be dangerous to
the person providing aid to give mouth-to-mouth
resuscitation.
Dpn I
No action shall be taken involving any personal risk
or without suitable training. It may be dangerous to
the person providing aid to give mouth-to-mouth
resuscitation.
Control Primer 1 (34-mer)
No action shall be taken involving any personal risk
or without suitable training.
Control Primer 2 (34-mer)
No action shall be taken involving any personal risk
or without suitable training.
pWS4.5 Control Template
No action shall be taken involving any personal risk
or without suitable training.
QuikChange XL dNTP Mix
No action shall be taken involving any personal risk
or without suitable training.
XL10-Gold Ultracompetent cells
No action shall be taken involving any personal risk
or without suitable training. It may be dangerous to
the person providing aid to give mouth-to-mouth
resuscitation.
XL10-Gold 2-Mercaptoethanol
No action shall be taken involving any personal risk
or without suitable training. If it is suspected that
fumes are still present, the rescuer should wear an
appropriate mask or self-contained breathing
apparatus. It may be dangerous to the person
providing aid to give mouth-to-mouth resuscitation.
Wash contaminated clothing thoroughly with water
before removing it, or wear gloves.
pUC 18 DNA Control Plasmid
No action shall be taken involving any personal risk
or without suitable training.
surveillance for 48 hours.
XL10-Gold Ultracompetent cells
Treat symptomatically. Contact poison treatment
specialist immediately if large quantities have been
ingested or inhaled.
XL10-Gold 2-Mercaptoethanol
Treat symptomatically. Contact poison treatment
specialist immediately if large quantities have been
ingested or inhaled.
pUC 18 DNA Control Plasmid
Treat symptomatically. Contact poison treatment
specialist immediately if large quantities have been
ingested or inhaled.
Specific treatments
:
QuikSolution
No specific treatment.
PfuUltra HF DNA Polymerase
No specific treatment.
10X Reaction Buffer
No specific treatment.
Dpn I
No specific treatment.
Control Primer 1 (34-mer)
No specific treatment.
Control Primer 2 (34-mer)
No specific treatment.
pWS4.5 Control Template
No specific treatment.
QuikChange XL dNTP Mix
No specific treatment.
XL10-Gold Ultracompetent cells
No specific treatment.
XL10-Gold 2-Mercaptoethanol
No specific treatment.
pUC 18 DNA Control Plasmid
No specific treatment.
15/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 4. First aid measures
See toxicological information (Section 11)
Section 5. Fire-fighting measures
Specific hazards arising
from the chemical
QuikSolution
Combustible liquid. Runoff to sewer may create fire
or explosion hazard. In a fire or if heated, a
pressure increase will occur and the container may
burst, with the risk of a subsequent explosion. The
vapor/gas is heavier than air and will spread along
the ground. Vapors may accumulate in low or
confined areas or travel a considerable distance to
a source of ignition and flash back.
PfuUltra HF DNA Polymerase
In a fire or if heated, a pressure increase will occur
and the container may burst.
10X Reaction Buffer
In a fire or if heated, a pressure increase will occur
and the container may burst. This material is
harmful to aquatic life with long lasting effects. Fire
water contaminated with this material must be
contained and prevented from being discharged to
any waterway, sewer or drain.
Dpn I
In a fire or if heated, a pressure increase will occur
and the container may burst.
Control Primer 1 (34-mer)
In a fire or if heated, a pressure increase will occur
QuikSolution
Use dry chemical,
CO₂,
water spray (fog) or foam.
PfuUltra HF DNA Polymerase
Use an extinguishing agent suitable for the
surrounding fire.
10X Reaction Buffer
Use an extinguishing agent suitable for the
surrounding fire.
Dpn I
Use an extinguishing agent suitable for the
surrounding fire.
Control Primer 1 (34-mer)
Use an extinguishing agent suitable for the
surrounding fire.
Control Primer 2 (34-mer)
Use an extinguishing agent suitable for the
surrounding fire.
pWS4.5 Control Template
Use an extinguishing agent suitable for the
surrounding fire.
QuikChange XL dNTP Mix
Use an extinguishing agent suitable for the
surrounding fire.
XL10-Gold Ultracompetent cells
Use an extinguishing agent suitable for the
surrounding fire.
XL10-Gold 2-Mercaptoethanol
Use an extinguishing agent suitable for the
surrounding fire.
pUC 18 DNA Control Plasmid
Use an extinguishing agent suitable for the
surrounding fire.
5.1 Extinguishing media
:
QuikSolution
Do not use water jet.
PfuUltra HF DNA Polymerase
None known.
10X Reaction Buffer
None known.
Dpn I
None known.
Control Primer 1 (34-mer)
None known.
Control Primer 2 (34-mer)
None known.
pWS4.5 Control Template
None known.
QuikChange XL dNTP Mix
None known.
XL10-Gold Ultracompetent cells
None known.
XL10-Gold 2-Mercaptoethanol
None known.
pUC 18 DNA Control Plasmid
None known.
Suitable extinguishing
media
:
Unsuitable extinguishing
media
:
5.2 Special hazards arising from the substance or mixture
16/52
Date of issue :
12/16/2022
QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 5. Fire-fighting measures
Hazardous thermal
decomposition products
QuikSolution
Decomposition products may include the following
materials:
carbon dioxide
carbon monoxide
sulfur oxides
PfuUltra HF DNA Polymerase
Decomposition products may include the following
materials:
carbon dioxide
carbon monoxide
10X Reaction Buffer
Decomposition products may include the following
materials:
carbon dioxide
carbon monoxide
nitrogen oxides
sulfur oxides
halogenated compounds
Dpn I
Decomposition products may include the following
materials:
carbon dioxide
carbon monoxide
halogenated compounds
metal oxide/oxides
Control Primer 1 (34-mer)
No specific data.
Control Primer 2 (34-mer)
No specific data.
pWS4.5 Control Template
No specific data.
QuikChange XL dNTP Mix
Decomposition products may include the following
materials:
carbon dioxide
carbon monoxide
nitrogen oxides
phosphorus oxides
XL10-Gold Ultracompetent cells
Decomposition products may include the following
materials:
carbon dioxide
carbon monoxide
sulfur oxides
halogenated compounds
metal oxide/oxides
XL10-Gold 2-Mercaptoethanol
Decomposition products may include the following
materials:
carbon dioxide
carbon monoxide
sulfur oxides
and the container may burst.
Control Primer 2 (34-mer)
In a fire or if heated, a pressure increase will occur
and the container may burst.
pWS4.5 Control Template
In a fire or if heated, a pressure increase will occur
and the container may burst.
QuikChange XL dNTP Mix
In a fire or if heated, a pressure increase will occur
and the container may burst.
XL10-Gold Ultracompetent cells
In a fire or if heated, a pressure increase will occur
and the container may burst.
XL10-Gold 2-Mercaptoethanol
In a fire or if heated, a pressure increase will occur
and the container may burst. This material is
harmful to aquatic life with long lasting effects. Fire
water contaminated with this material must be
contained and prevented from being discharged to
any waterway, sewer or drain.
pUC 18 DNA Control Plasmid
In a fire or if heated, a pressure increase will occur
and the container may burst.
:
17/52
Date of issue :
12/16/2022
QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 5. Fire-fighting measures
QuikSolution
Promptly isolate the scene by removing all persons
from the vicinity of the incident if there is a fire. No
action shall be taken involving any personal risk or
without suitable training. Move containers from fire
area if this can be done without risk. Use water
spray to keep fire-exposed containers cool.
PfuUltra HF DNA Polymerase
Promptly isolate the scene by removing all persons
from the vicinity of the incident if there is a fire. No
action shall be taken involving any personal risk or
without suitable training.
10X Reaction Buffer
Promptly isolate the scene by removing all persons
from the vicinity of the incident if there is a fire. No
action shall be taken involving any personal risk or
without suitable training.
Dpn I
Promptly isolate the scene by removing all persons
from the vicinity of the incident if there is a fire. No
action shall be taken involving any personal risk or
without suitable training.
Control Primer 1 (34-mer)
Promptly isolate the scene by removing all persons
from the vicinity of the incident if there is a fire. No
action shall be taken involving any personal risk or
without suitable training.
Control Primer 2 (34-mer)
Promptly isolate the scene by removing all persons
from the vicinity of the incident if there is a fire. No
action shall be taken involving any personal risk or
without suitable training.
pWS4.5 Control Template
Promptly isolate the scene by removing all persons
from the vicinity of the incident if there is a fire. No
action shall be taken involving any personal risk or
without suitable training.
QuikChange XL dNTP Mix
Promptly isolate the scene by removing all persons
from the vicinity of the incident if there is a fire. No
action shall be taken involving any personal risk or
without suitable training.
XL10-Gold Ultracompetent cells
Promptly isolate the scene by removing all persons
from the vicinity of the incident if there is a fire. No
action shall be taken involving any personal risk or
without suitable training.
XL10-Gold 2-Mercaptoethanol
Promptly isolate the scene by removing all persons
from the vicinity of the incident if there is a fire. No
action shall be taken involving any personal risk or
without suitable training.
pUC 18 DNA Control Plasmid
Promptly isolate the scene by removing all persons
from the vicinity of the incident if there is a fire. No
action shall be taken involving any personal risk or
without suitable training.
halogenated compounds
metal oxide/oxides
pUC 18 DNA Control Plasmid
No specific data.
QuikSolution
Fire-fighters should wear appropriate protective
equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive
pressure mode.
PfuUltra HF DNA Polymerase
Fire-fighters should wear appropriate protective
equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive
pressure mode.
Special protective
equipment for fire-fighters
:
Special protective actions
for fire-fighters
:
5.3 Advice for firefighters
18/52
Date of issue :
12/16/2022
QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 5. Fire-fighting measures
10X Reaction Buffer
Fire-fighters should wear appropriate protective
equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive
pressure mode.
Dpn I
Fire-fighters should wear appropriate protective
equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive
pressure mode.
Control Primer 1 (34-mer)
Fire-fighters should wear appropriate protective
equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive
pressure mode.
Control Primer 2 (34-mer)
Fire-fighters should wear appropriate protective
equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive
pressure mode.
pWS4.5 Control Template
Fire-fighters should wear appropriate protective
equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive
pressure mode.
QuikChange XL dNTP Mix
Fire-fighters should wear appropriate protective
equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive
pressure mode.
XL10-Gold Ultracompetent cells
Fire-fighters should wear appropriate protective
equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive
pressure mode.
XL10-Gold 2-Mercaptoethanol
Fire-fighters should wear appropriate protective
equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive
pressure mode.
pUC 18 DNA Control Plasmid
Fire-fighters should wear appropriate protective
equipment and self-contained breathing apparatus
(SCBA) with a full face-piece operated in positive
pressure mode.
Section 6. Accidental release measures
6.1 Personal precautions, protective equipment and emergency procedures
:
QuikSolution
No action shall be taken involving any personal
risk or without suitable training. Evacuate
surrounding areas. Keep unnecessary and
unprotected personnel from entering. Do not
touch or walk through spilled material. Shut off all
ignition sources. No flares, smoking or flames in
hazard area. Avoid breathing vapor or mist.
Provide adequate ventilation. Wear appropriate
respirator when ventilation is inadequate. Put on
appropriate personal protective equipment.
PfuUltra HF DNA Polymerase
No action shall be taken involving any personal
risk or without suitable training. Evacuate
surrounding areas. Keep unnecessary and
unprotected personnel from entering. Do not
touch or walk through spilled material. Avoid
breathing vapor or mist. Provide adequate
ventilation. Wear appropriate respirator when
ventilation is inadequate. Put on appropriate
personal protective equipment.
10X Reaction Buffer
No action shall be taken involving any personal
For non-emergency
personnel
19/52
Date of issue :
12/16/2022
QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 6. Accidental release measures
risk or without suitable training. Evacuate
surrounding areas. Keep unnecessary and
unprotected personnel from entering. Do not
touch or walk through spilled material. Avoid
breathing vapor or mist. Provide adequate
ventilation. Wear appropriate respirator when
ventilation is inadequate. Put on appropriate
personal protective equipment.
Dpn I
No action shall be taken involving any personal
risk or without suitable training. Evacuate
surrounding areas. Keep unnecessary and
unprotected personnel from entering. Do not
touch or walk through spilled material. Avoid
breathing vapor or mist. Provide adequate
ventilation. Wear appropriate respirator when
ventilation is inadequate. Put on appropriate
personal protective equipment.
Control Primer 1 (34-mer)
No action shall be taken involving any personal
risk or without suitable training. Evacuate
surrounding areas. Keep unnecessary and
unprotected personnel from entering. Do not
touch or walk through spilled material. Put on
appropriate personal protective equipment.
Control Primer 2 (34-mer)
No action shall be taken involving any personal
risk or without suitable training. Evacuate
surrounding areas. Keep unnecessary and
unprotected personnel from entering. Do not
touch or walk through spilled material. Put on
appropriate personal protective equipment.
pWS4.5 Control Template
No action shall be taken involving any personal
risk or without suitable training. Evacuate
surrounding areas. Keep unnecessary and
unprotected personnel from entering. Do not
touch or walk through spilled material. Put on
appropriate personal protective equipment.
QuikChange XL dNTP Mix
No action shall be taken involving any personal
risk or without suitable training. Evacuate
surrounding areas. Keep unnecessary and
unprotected personnel from entering. Do not
touch or walk through spilled material. Put on
appropriate personal protective equipment.
XL10-Gold Ultracompetent cells
No action shall be taken involving any personal
risk or without suitable training. Evacuate
surrounding areas. Keep unnecessary and
unprotected personnel from entering. Do not
touch or walk through spilled material. Avoid
breathing vapor or mist. Provide adequate
ventilation. Wear appropriate respirator when
ventilation is inadequate. Put on appropriate
personal protective equipment.
XL10-Gold 2-Mercaptoethanol
No action shall be taken involving any personal
risk or without suitable training. Evacuate
surrounding areas. Keep unnecessary and
unprotected personnel from entering. Do not
touch or walk through spilled material. Do not
breathe vapor or mist. Provide adequate
ventilation. Wear appropriate respirator when
ventilation is inadequate. Put on appropriate
personal protective equipment.
pUC 18 DNA Control Plasmid
No action shall be taken involving any personal
20/52
Date of issue :
12/16/2022
QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 6. Accidental release measures
6.2 Environmental
precautions
:
risk or without suitable training. Evacuate
surrounding areas. Keep unnecessary and
unprotected personnel from entering. Do not
touch or walk through spilled material. Put on
appropriate personal protective equipment.
QuikSolution
Avoid dispersal of spilled material and runoff and
contact with soil, waterways, drains and sewers.
Inform the relevant authorities if the product has
caused environmental pollution (sewers,
waterways, soil or air).
PfuUltra HF DNA Polymerase
Avoid dispersal of spilled material and runoff and
contact with soil, waterways, drains and sewers.
Inform the relevant authorities if the product has
caused environmental pollution (sewers,
waterways, soil or air).
For emergency responders
:
QuikSolution
If specialized clothing is required to deal with the
spillage, take note of any information in Section 8
on suitable and unsuitable materials. See also
the information in "For non-emergency personnel".
PfuUltra HF DNA Polymerase
If specialized clothing is required to deal with the
spillage, take note of any information in Section 8
on suitable and unsuitable materials. See also
the information in "For non-emergency personnel".
10X Reaction Buffer
If specialized clothing is required to deal with the
spillage, take note of any information in Section 8
on suitable and unsuitable materials. See also
the information in "For non-emergency personnel".
Dpn I
If specialized clothing is required to deal with the
spillage, take note of any information in Section 8
on suitable and unsuitable materials. See also
the information in "For non-emergency personnel".
Control Primer 1 (34-mer)
If specialized clothing is required to deal with the
spillage, take note of any information in Section 8
on suitable and unsuitable materials. See also
the information in "For non-emergency personnel".
Control Primer 2 (34-mer)
If specialized clothing is required to deal with the
spillage, take note of any information in Section 8
on suitable and unsuitable materials. See also
the information in "For non-emergency personnel".
pWS4.5 Control Template
If specialized clothing is required to deal with the
spillage, take note of any information in Section 8
on suitable and unsuitable materials. See also
the information in "For non-emergency personnel".
QuikChange XL dNTP Mix
If specialized clothing is required to deal with the
spillage, take note of any information in Section 8
on suitable and unsuitable materials. See also
the information in "For non-emergency personnel".
XL10-Gold Ultracompetent cells
If specialized clothing is required to deal with the
spillage, take note of any information in Section 8
on suitable and unsuitable materials. See also
the information in "For non-emergency personnel".
XL10-Gold 2-Mercaptoethanol
If specialized clothing is required to deal with the
spillage, take note of any information in Section 8
on suitable and unsuitable materials. See also
the information in "For non-emergency personnel".
pUC 18 DNA Control Plasmid
If specialized clothing is required to deal with the
spillage, take note of any information in Section 8
on suitable and unsuitable materials. See also
the information in "For non-emergency personnel".
21/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 6. Accidental release measures
10X Reaction Buffer
Avoid dispersal of spilled material and runoff and
contact with soil, waterways, drains and sewers.
Inform the relevant authorities if the product has
caused environmental pollution (sewers,
waterways, soil or air). Water polluting material.
May be harmful to the environment if released in
large quantities.
Dpn I
Avoid dispersal of spilled material and runoff and
contact with soil, waterways, drains and sewers.
Inform the relevant authorities if the product has
caused environmental pollution (sewers,
waterways, soil or air).
Control Primer 1 (34-mer)
Avoid dispersal of spilled material and runoff and
contact with soil, waterways, drains and sewers.
Inform the relevant authorities if the product has
caused environmental pollution (sewers,
waterways, soil or air).
Control Primer 2 (34-mer)
Avoid dispersal of spilled material and runoff and
contact with soil, waterways, drains and sewers.
Inform the relevant authorities if the product has
caused environmental pollution (sewers,
waterways, soil or air).
pWS4.5 Control Template
Avoid dispersal of spilled material and runoff and
contact with soil, waterways, drains and sewers.
Inform the relevant authorities if the product has
caused environmental pollution (sewers,
waterways, soil or air).
QuikChange XL dNTP Mix
Avoid dispersal of spilled material and runoff and
contact with soil, waterways, drains and sewers.
Inform the relevant authorities if the product has
caused environmental pollution (sewers,
waterways, soil or air).
XL10-Gold Ultracompetent cells
Avoid dispersal of spilled material and runoff and
contact with soil, waterways, drains and sewers.
Inform the relevant authorities if the product has
caused environmental pollution (sewers,
waterways, soil or air).
XL10-Gold 2-Mercaptoethanol
Avoid dispersal of spilled material and runoff and
contact with soil, waterways, drains and sewers.
Inform the relevant authorities if the product has
caused environmental pollution (sewers,
waterways, soil or air). Water polluting material.
May be harmful to the environment if released in
large quantities.
pUC 18 DNA Control Plasmid
Avoid dispersal of spilled material and runoff and
contact with soil, waterways, drains and sewers.
Inform the relevant authorities if the product has
caused environmental pollution (sewers,
waterways, soil or air).
QuikSolution
Stop leak if without risk. Move containers from spill
area. Use spark-proof tools and explosion-proof
equipment. Dilute with water and mop up if water-
soluble. Alternatively, or if water-insoluble, absorb
with an inert dry material and place in an
appropriate waste disposal container. Dispose of
via a licensed waste disposal contractor.
PfuUltra HF DNA Polymerase
Stop leak if without risk. Move containers from spill
Methods for cleaning up
:
6.3 Methods and materials for containment and cleaning up
22/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 6. Accidental release measures
area. Dilute with water and mop up if water-soluble.
Alternatively, or if water-insoluble, absorb with an
inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste
disposal contractor.
10X Reaction Buffer
Stop leak if without risk. Move containers from spill
area. Dilute with water and mop up if water-soluble.
Alternatively, or if water-insoluble, absorb with an
inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste
disposal contractor.
Dpn I
Stop leak if without risk. Move containers from spill
area. Dilute with water and mop up if water-soluble.
Alternatively, or if water-insoluble, absorb with an
inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste
disposal contractor.
Control Primer 1 (34-mer)
Stop leak if without risk. Move containers from spill
area. Dilute with water and mop up if water-soluble.
Alternatively, or if water-insoluble, absorb with an
inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste
disposal contractor.
Control Primer 2 (34-mer)
Stop leak if without risk. Move containers from spill
area. Dilute with water and mop up if water-soluble.
Alternatively, or if water-insoluble, absorb with an
inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste
disposal contractor.
pWS4.5 Control Template
Stop leak if without risk. Move containers from spill
area. Dilute with water and mop up if water-soluble.
Alternatively, or if water-insoluble, absorb with an
inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste
disposal contractor.
QuikChange XL dNTP Mix
Stop leak if without risk. Move containers from spill
area. Dilute with water and mop up if water-soluble.
Alternatively, or if water-insoluble, absorb with an
inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste
disposal contractor.
XL10-Gold Ultracompetent cells
Stop leak if without risk. Move containers from spill
area. Dilute with water and mop up if water-soluble.
Alternatively, or if water-insoluble, absorb with an
inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste
disposal contractor.
XL10-Gold 2-Mercaptoethanol
Stop leak if without risk. Move containers from spill
area. Dilute with water and mop up if water-soluble.
Alternatively, or if water-insoluble, absorb with an
inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste
disposal contractor.
pUC 18 DNA Control Plasmid
Stop leak if without risk. Move containers from spill
area. Dilute with water and mop up if water-soluble.
Alternatively, or if water-insoluble, absorb with an
inert dry material and place in an appropriate waste
disposal container. Dispose of via a licensed waste
disposal contractor.
23/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 7. Handling and storage
Protective measures
QuikSolution
Put on appropriate personal protective equipment
(see Section 8). Do not ingest. Avoid contact with
eyes, skin and clothing. Avoid breathing vapor or
mist. Use only with adequate ventilation. Wear
appropriate respirator when ventilation is
inadequate. Do not enter storage areas and
confined spaces unless adequately ventilated.
Keep in the original container or an approved
alternative made from a compatible material, kept
tightly closed when not in use. Store and use away
from heat, sparks, open flame or any other ignition
source. Use explosion-proof electrical (ventilating,
lighting and material handling) equipment. Use
only non-sparking tools. Empty containers retain
product residue and can be hazardous. Do not
reuse container.
PfuUltra HF DNA Polymerase
Put on appropriate personal protective equipment
(see Section 8). Do not ingest. Avoid contact with
eyes, skin and clothing. Avoid breathing vapor or
mist. Keep in the original container or an approved
alternative made from a compatible material, kept
tightly closed when not in use. Empty containers
retain product residue and can be hazardous. Do
not reuse container.
10X Reaction Buffer
Put on appropriate personal protective equipment
(see Section 8). Do not ingest. Avoid contact with
eyes, skin and clothing. Avoid breathing vapor or
mist. Avoid release to the environment. Keep in
the original container or an approved alternative
made from a compatible material, kept tightly
closed when not in use. Empty containers retain
product residue and can be hazardous. Do not
reuse container.
Dpn I
Put on appropriate personal protective equipment
(see Section 8). Do not ingest. Avoid contact with
eyes, skin and clothing. Avoid breathing vapor or
mist. Keep in the original container or an approved
alternative made from a compatible material, kept
tightly closed when not in use. Empty containers
retain product residue and can be hazardous. Do
not reuse container.
Control Primer 1 (34-mer)
Put on appropriate personal protective equipment
(see Section 8).
Control Primer 2 (34-mer)
Put on appropriate personal protective equipment
(see Section 8).
pWS4.5 Control Template
Put on appropriate personal protective equipment
(see Section 8).
QuikChange XL dNTP Mix
Put on appropriate personal protective equipment
(see Section 8).
XL10-Gold Ultracompetent cells
Put on appropriate personal protective equipment
(see Section 8). Do not ingest. Avoid contact with
eyes, skin and clothing. Avoid breathing vapor or
mist. Keep in the original container or an approved
alternative made from a compatible material, kept
tightly closed when not in use. Empty containers
retain product residue and can be hazardous. Do
not reuse container.
XL10-Gold 2-Mercaptoethanol
Put on appropriate personal protective equipment
:
7.1 Precautions for safe handling
24/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 7. Handling and storage
Advice on general
occupational hygiene
QuikSolution
Eating, drinking and smoking should be prohibited
in areas where this material is handled, stored and
processed. Workers should wash hands and face
before eating, drinking and smoking. Remove
contaminated clothing and protective equipment
before entering eating areas. See also Section 8
for additional information on hygiene measures.
PfuUltra HF DNA Polymerase
Eating, drinking and smoking should be prohibited
in areas where this material is handled, stored and
processed. Workers should wash hands and face
before eating, drinking and smoking. Remove
contaminated clothing and protective equipment
before entering eating areas. See also Section 8
for additional information on hygiene measures.
10X Reaction Buffer
Eating, drinking and smoking should be prohibited
in areas where this material is handled, stored and
processed. Workers should wash hands and face
before eating, drinking and smoking. Remove
contaminated clothing and protective equipment
before entering eating areas. See also Section 8
for additional information on hygiene measures.
Dpn I
Eating, drinking and smoking should be prohibited
in areas where this material is handled, stored and
processed. Workers should wash hands and face
before eating, drinking and smoking. Remove
contaminated clothing and protective equipment
before entering eating areas. See also Section 8
for additional information on hygiene measures.
Control Primer 1 (34-mer)
Eating, drinking and smoking should be prohibited
in areas where this material is handled, stored and
processed. Workers should wash hands and face
before eating, drinking and smoking. Remove
contaminated clothing and protective equipment
before entering eating areas. See also Section 8
for additional information on hygiene measures.
Control Primer 2 (34-mer)
Eating, drinking and smoking should be prohibited
in areas where this material is handled, stored and
processed. Workers should wash hands and face
before eating, drinking and smoking. Remove
contaminated clothing and protective equipment
before entering eating areas. See also Section 8
for additional information on hygiene measures.
:
(see Section 8). Persons with a history of skin
sensitization problems should not be employed in
any process in which this product is used. Avoid
exposure - obtain special instructions before use.
Avoid exposure during pregnancy. Do not handle
until all safety precautions have been read and
understood. Do not get in eyes or on skin or
clothing. Do not breathe vapor or mist. Do not
ingest. Avoid release to the environment. If during
normal use the material presents a respiratory
hazard, use only with adequate ventilation or wear
appropriate respirator. Keep in the original
container or an approved alternative made from a
compatible material, kept tightly closed when not in
use. Empty containers retain product residue and
can be hazardous. Do not reuse container.
pUC 18 DNA Control Plasmid
Put on appropriate personal protective equipment
(see Section 8).
25/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 7. Handling and storage
7.2 Conditions for safe
storage, including any
incompatibilities
pWS4.5 Control Template
Eating, drinking and smoking should be prohibited
in areas where this material is handled, stored and
processed. Workers should wash hands and face
before eating, drinking and smoking. Remove
contaminated clothing and protective equipment
before entering eating areas. See also Section 8
for additional information on hygiene measures.
QuikChange XL dNTP Mix
Eating, drinking and smoking should be prohibited
in areas where this material is handled, stored and
processed. Workers should wash hands and face
before eating, drinking and smoking. Remove
contaminated clothing and protective equipment
before entering eating areas. See also Section 8
for additional information on hygiene measures.
XL10-Gold Ultracompetent cells
Potentially biohazardous material. Eating, drinking
and smoking should be prohibited in areas where
this material is handled, stored and processed.
Workers should wash hands and face before eating,
drinking and smoking. Remove contaminated
clothing and protective equipment before entering
eating areas. See also Section 8 for additional
information on hygiene measures.
XL10-Gold 2-Mercaptoethanol
Eating, drinking and smoking should be prohibited
in areas where this material is handled, stored and
processed. Workers should wash hands and face
before eating, drinking and smoking. Remove
contaminated clothing and protective equipment
before entering eating areas. See also Section 8
for additional information on hygiene measures.
pUC 18 DNA Control Plasmid
Eating, drinking and smoking should be prohibited
in areas where this material is handled, stored and
processed. Workers should wash hands and face
before eating, drinking and smoking. Remove
contaminated clothing and protective equipment
before entering eating areas. See also Section 8
for additional information on hygiene measures.
QuikSolution
Store in accordance with local regulations. Store in
a segregated and approved area. Store in original
container protected from direct sunlight in a dry,
cool and well-ventilated area, away from
incompatible materials (see Section 10) and food
and drink. Eliminate all ignition sources. Separate
from oxidizing materials. Keep container tightly
closed and sealed until ready for use. Containers
that have been opened must be carefully resealed
and kept upright to prevent leakage. Do not store
in unlabeled containers. Use appropriate
containment to avoid environmental contamination.
See Section 10 for incompatible materials before
handling or use.
PfuUltra HF DNA Polymerase
Store in accordance with local regulations. Store in
original container protected from direct sunlight in a
dry, cool and well-ventilated area, away from
incompatible materials (see Section 10) and food
and drink. Keep container tightly closed and sealed
until ready for use. Containers that have been
opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled
containers. Use appropriate containment to avoid
:
26/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 7. Handling and storage
environmental contamination. See Section 10 for
incompatible materials before handling or use.
10X Reaction Buffer
Store in accordance with local regulations. Store in
original container protected from direct sunlight in a
dry, cool and well-ventilated area, away from
incompatible materials (see Section 10) and food
and drink. Keep container tightly closed and sealed
until ready for use. Containers that have been
opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled
containers. Use appropriate containment to avoid
environmental contamination. See Section 10 for
incompatible materials before handling or use.
Dpn I
Store in accordance with local regulations. Store in
original container protected from direct sunlight in a
dry, cool and well-ventilated area, away from
incompatible materials (see Section 10) and food
and drink. Keep container tightly closed and sealed
until ready for use. Containers that have been
opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled
containers. Use appropriate containment to avoid
environmental contamination. See Section 10 for
incompatible materials before handling or use.
Control Primer 1 (34-mer)
Store in accordance with local regulations. Store in
original container protected from direct sunlight in a
dry, cool and well-ventilated area, away from
incompatible materials (see Section 10) and food
and drink. Keep container tightly closed and sealed
until ready for use. Containers that have been
opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled
containers. Use appropriate containment to avoid
environmental contamination. See Section 10 for
incompatible materials before handling or use.
Control Primer 2 (34-mer)
Store in accordance with local regulations. Store in
original container protected from direct sunlight in a
dry, cool and well-ventilated area, away from
incompatible materials (see Section 10) and food
and drink. Keep container tightly closed and sealed
until ready for use. Containers that have been
opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled
containers. Use appropriate containment to avoid
environmental contamination. See Section 10 for
incompatible materials before handling or use.
pWS4.5 Control Template
Store in accordance with local regulations. Store in
original container protected from direct sunlight in a
dry, cool and well-ventilated area, away from
incompatible materials (see Section 10) and food
and drink. Keep container tightly closed and sealed
until ready for use. Containers that have been
opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled
containers. Use appropriate containment to avoid
environmental contamination. See Section 10 for
incompatible materials before handling or use.
QuikChange XL dNTP Mix
Store in accordance with local regulations. Store in
original container protected from direct sunlight in a
dry, cool and well-ventilated area, away from
27/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 7. Handling and storage
incompatible materials (see Section 10) and food
and drink. Keep container tightly closed and sealed
until ready for use. Containers that have been
opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled
containers. Use appropriate containment to avoid
environmental contamination. See Section 10 for
incompatible materials before handling or use.
XL10-Gold Ultracompetent cells
Store in accordance with local regulations. Store in
original container protected from direct sunlight in a
dry, cool and well-ventilated area, away from
incompatible materials (see Section 10) and food
and drink. Keep container tightly closed and sealed
until ready for use. Containers that have been
opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled
containers. Use appropriate containment to avoid
environmental contamination. See Section 10 for
incompatible materials before handling or use.
XL10-Gold 2-Mercaptoethanol
Store in accordance with local regulations. Store in
original container protected from direct sunlight in a
dry, cool and well-ventilated area, away from
incompatible materials (see Section 10) and food
and drink. Store locked up. Keep container tightly
closed and sealed until ready for use. Containers
that have been opened must be carefully resealed
and kept upright to prevent leakage. Do not store
in unlabeled containers. Use appropriate
containment to avoid environmental contamination.
See Section 10 for incompatible materials before
handling or use.
pUC 18 DNA Control Plasmid
Store in accordance with local regulations. Store in
original container protected from direct sunlight in a
dry, cool and well-ventilated area, away from
incompatible materials (see Section 10) and food
and drink. Keep container tightly closed and sealed
until ready for use. Containers that have been
opened must be carefully resealed and kept upright
to prevent leakage. Do not store in unlabeled
containers. Use appropriate containment to avoid
environmental contamination. See Section 10 for
incompatible materials before handling or use.
Recommendations
:
QuikSolution
Industrial applications, Professional applications.
PfuUltra HF DNA Polymerase
Industrial applications, Professional applications.
10X Reaction Buffer
Industrial applications, Professional applications.
Dpn I
Industrial applications, Professional applications.
Control Primer 1 (34-mer)
Industrial applications, Professional applications.
Control Primer 2 (34-mer)
Industrial applications, Professional applications.
pWS4.5 Control Template
Industrial applications, Professional applications.
QuikChange XL dNTP Mix
Industrial applications, Professional applications.
XL10-Gold Ultracompetent cells
Industrial applications, Professional applications.
XL10-Gold 2-Mercaptoethanol
Industrial applications, Professional applications.
pUC 18 DNA Control Plasmid
Industrial applications, Professional applications.
7.3 Specific end use(s)
28/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 7. Handling and storage
:
Industrial sector specific
solutions
QuikSolution
Not available.
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
QuikSolution
Dimethyl sulfoxide
OARS WEEL (United States, 1/2021).
TWA: 250 ppm 8 hours.
PfuUltra HF DNA Polymerase
Glycerol
OSHA PEL 1989 (United States, 3/1989).
TWA: 5 mg/m³ 8 hours. Form: Respirable
fraction
TWA: 10 mg/m³ 8 hours. Form: Total dust
OSHA PEL (United States, 5/2018).
TWA: 5 mg/m³ 8 hours. Form: Respirable
fraction
TWA: 15 mg/m³ 8 hours. Form: Total dust
Poly(oxy-1,2-ethanediyl), .alpha.-[(1,1,3,3-tetramethylbutyl)phenyl]-.
omega.-hydroxy-
None.
10X Reaction Buffer
Ammonium sulphate
None.
Polyoxyethylene octyl phenyl ether
None.
Dpn I
Glycerol
OSHA PEL 1989 (United States, 3/1989).
TWA: 5 mg/m³ 8 hours. Form: Respirable
fraction
TWA: 10 mg/m³ 8 hours. Form: Total dust
OSHA PEL (United States, 5/2018).
TWA: 5 mg/m³ 8 hours. Form: Respirable
fraction
TWA: 15 mg/m³ 8 hours. Form: Total dust
XL10-Gold Ultracompetent cells
Glycerol
OSHA PEL 1989 (United States, 3/1989).
TWA: 5 mg/m³ 8 hours. Form: Respirable
fraction
TWA: 10 mg/m³ 8 hours. Form: Total dust
OSHA PEL (United States, 5/2018).
TWA: 5 mg/m³ 8 hours. Form: Respirable
fraction
TWA: 15 mg/m³ 8 hours. Form: Total dust
Dimethyl sulfoxide
OARS WEEL (United States, 1/2021).
TWA: 250 ppm 8 hours.
Section 8. Exposure controls/personal protection
Ingredient name
Exposure limits
8.1 Control parameters
Occupational exposure limits
29/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 8. Exposure controls/personal protection
Potassium chloride
None.
XL10-Gold 2-Mercaptoethanol
2-Mercaptoethanol
OARS WEEL (United States, 1/2021).
Absorbed through skin.
TWA: 0.2 ppm 8 hours.
Hand protection
Based on the hazard and potential for exposure, select a respirator that meets the
appropriate standard or certification. Respirators must be used according to a
respiratory protection program to ensure proper fitting, training, and other important
aspects of use.
Chemical-resistant, impervious gloves complying with an approved standard should be
worn at all times when handling chemical products if a risk assessment indicates this is
necessary. Considering the parameters specified by the glove manufacturer, check
during use that the gloves are still retaining their protective properties. It should be
noted that the time to breakthrough for any glove material may be different for different
glove manufacturers. In the case of mixtures, consisting of several substances, the
protection time of the gloves cannot be accurately estimated.
Safety eyewear complying with an approved standard should be used when a risk
assessment indicates this is necessary to avoid exposure to liquid splashes, mists,
gases or dusts. If contact is possible, the following protection should be worn, unless
the assessment indicates a higher degree of protection: chemical splash goggles.
Eye/face protection
Respiratory protection
:
:
:
Body protection
Personal protective equipment for the body should be selected based on the task being
performed and the risks involved and should be approved by a specialist before
handling this product.
:
Environmental exposure
controls
:
Emissions from ventilation or work process equipment should be checked to ensure
they comply with the requirements of environmental protection legislation. In some
cases, fume scrubbers, filters or engineering modifications to the process equipment
will be necessary to reduce emissions to acceptable levels.
Appropriate engineering
controls
:
If user operations generate dust, fumes, gas, vapor or mist, use process enclosures,
local exhaust ventilation or other engineering controls to keep worker exposure to
airborne contaminants below any recommended or statutory limits.
Handle as biohazard material (Biosafety level 1). Wash hands, forearms and face
thoroughly after handling chemical products, before eating, smoking and using the
lavatory and at the end of the working period. Appropriate techniques should be used to
remove potentially contaminated clothing. Contaminated work clothing should not be
allowed out of the workplace. Wash contaminated clothing before reusing. Ensure that
eyewash stations and safety showers are close to the workstation location.
Hygiene measures
:
Individual protection measures
Skin protection
Other skin protection
:
Appropriate footwear and any additional skin protection measures should be selected
based on the task being performed and the risks involved and should be approved by a
specialist before handling this product.
8.2 Exposure controls
Biological exposure indices
No exposure indices known.
30/52
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 9. Physical and chemical properties and safety characteristics
Physical state
QuikSolution
Liquid. [Clear.]
PfuUltra HF DNA Polymerase
Liquid.
10X Reaction Buffer
Liquid.
Dpn I
Liquid.
Control Primer 1 (34-mer)
Liquid.
Control Primer 2 (34-mer)
Liquid.
pWS4.5 Control Template
Liquid.
QuikChange XL dNTP Mix
Liquid.
XL10-Gold Ultracompetent cells
Liquid.
XL10-Gold 2-Mercaptoethanol
Liquid.
pUC 18 DNA Control Plasmid
Liquid.
QuikSolution
Slight [Slight]
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
Odor
pH
QuikSolution
Colorless.
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
Color
QuikSolution
Not available.
PfuUltra HF DNA Polymerase
8.2
10X Reaction Buffer
8.8
Dpn I
Not available.
Control Primer 1 (34-mer)
7.5
Control Primer 2 (34-mer)
7.5
pWS4.5 Control Template
7.5
QuikChange XL dNTP Mix
7.5
XL10-Gold Ultracompetent cells
6.4
XL10-Gold 2-Mercaptoethanol
Not available.
QuikSolution
Not available.
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
Odor threshold
:
:
:
:
:
Appearance
The conditions of measurement of all properties are at standard temperature and pressure unless otherwise indicated.
31/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 9. Physical and chemical properties and safety characteristics
Melting point/freezing point
QuikSolution
18.4°C (65.1°F)
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
0°C (32°F)
Control Primer 2 (34-mer)
0°C (32°F)
pWS4.5 Control Template
0°C (32°F)
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
0°C (32°F)
Flash point
QuikSolution
Closed cup: 85°C (185°F) [ASTM D 93]
Open cup: 87°C (188.6°F)
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
pUC 18 DNA Control Plasmid
7.5
:
:
Boiling point, initial boiling
point, and boiling range
:
QuikSolution
188.8°C (371.8°F)
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
100°C (212°F)
Control Primer 2 (34-mer)
100°C (212°F)
pWS4.5 Control Template
100°C (212°F)
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
100°C (212°F)
Ingredient name
Closed cup
°C
°F
Method
Open cup
°C
°F
Method
PfuUltra HF DNA
Polymerase
Glycerol
177
350.6
10X Reaction
Buffer
Polyoxyethylene
octyl phenyl ether
251
483.8
Dpn I
Glycerol
177
350.6
XL10-Gold
Ultracompetent
cells
32/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 9. Physical and chemical properties and safety characteristics
Vapor pressure
QuikSolution
0.056 kPa (0.42 mm Hg) [EU A.4]
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
Evaporation rate
QuikSolution
0.026 (butyl acetate = 1)
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
:
:
Flammability
:
QuikSolution
Not applicable.
PfuUltra HF DNA Polymerase
Not applicable.
10X Reaction Buffer
Not applicable.
Dpn I
Not applicable.
Control Primer 1 (34-mer)
Not applicable.
Control Primer 2 (34-mer)
Not applicable.
pWS4.5 Control Template
Not applicable.
QuikChange XL dNTP Mix
Not applicable.
XL10-Gold Ultracompetent cells
Not applicable.
XL10-Gold 2-Mercaptoethanol
Not applicable.
pUC 18 DNA Control Plasmid
Not applicable.
Lower and upper explosion
limit/flammability limit
:
QuikSolution
Lower: 2.6%
Upper: 42%
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
Dimethyl sulfoxide
87
188.6
ASTM D 93
87
188.6
Glycerol
177
350.6
XL10-Gold
2-Mercaptoethanol
2-Mercaptoethanol
74
165.2
74
165.2
33/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 9. Physical and chemical properties and safety characteristics
Ingredient name
Vapor Pressure at 20˚C
Vapor pressure at 50˚C
mm Hg
kPa
Method
mm
Hg
kPa
Method
PfuUltra HF DNA
Polymerase
water
23.8
3.2
92.258
12.3
Glycerol
0.000075
0.00001
0.0025
0.00033
10X Reaction
Buffer
water
23.8
3.2
92.258
12.3
Polyoxyethylene
octyl phenyl ether
0.997581
0.13
Dpn I
water
23.8
3.2
92.258
12.3
Glycerol
0.000075
0.00001
0.0025
0.00033
Control Primer 1
(34-mer)
water
23.8
3.2
92.258
12.3
Control Primer 2
(34-mer)
water
23.8
3.2
92.258
12.3
pWS4.5 Control
Template
water
23.8
3.2
92.258
12.3
QuikChange XL
dNTP Mix
water
23.8
3.2
92.258
12.3
XL10-Gold
Ultracompetent
cells
water
23.8
3.2
92.258
12.3
Dimethyl sulfoxide
0.42
0.056
EU A.4
XL10-Gold
34/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 9. Physical and chemical properties and safety characteristics
Relative density
Relative vapor density
QuikSolution
1.1
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
QuikSolution
2.71 [Air = 1]
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
:
:
2-Mercaptoethanol
water
23.8
3.2
92.258
12.3
2-Mercaptoethanol
0.98
0.13
pUC 18 DNA
Control Plasmid
water
23.8
3.2
92.258
12.3
Solubility(ies)
:
Media
Result
QuikSolution
water
Soluble
PfuUltra HF DNA
Polymerase
water
Soluble
10X Reaction Buffer
water
Soluble
Dpn I
water
Soluble
Control Primer 1
(34-mer)
water
Soluble
Control Primer 2
(34-mer)
water
Soluble
pWS4.5 Control
Template
water
Soluble
QuikChange XL dNTP
Mix
water
Soluble
XL10-Gold
Ultracompetent cells
water
Soluble
35/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 9. Physical and chemical properties and safety characteristics
Auto-ignition temperature
QuikSolution
300 to 302°C (572 to 575.6°F)
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
QuikSolution
-2.029
PfuUltra HF DNA Polymerase
Not applicable.
10X Reaction Buffer
Not applicable.
Dpn I
Not applicable.
Control Primer 1 (34-mer)
Not applicable.
Control Primer 2 (34-mer)
Not applicable.
pWS4.5 Control Template
Not applicable.
QuikChange XL dNTP Mix
Not applicable.
XL10-Gold Ultracompetent cells
Not applicable.
XL10-Gold 2-Mercaptoethanol
Not applicable.
pUC 18 DNA Control Plasmid
Not applicable.
Partition coefficient: n-
octanol/water
:
:
Decomposition temperature
:
QuikSolution
140 to 189°C (284 to 372.2°F)
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
Ingredient name
°C
°F
Method
PfuUltra HF DNA Polymerase
Glycerol
370
698
Dpn I
Glycerol
370
698
XL10-Gold Ultracompetent
cells
Dimethyl sulfoxide
300 to 302
572 to 575.6
Glycerol
370
698
XL10-Gold 2-Mercaptoethanol
2-Mercaptoethanol
295
563
XL10-Gold
2-Mercaptoethanol
water
Soluble
pUC 18 DNA Control
Plasmid
water
Soluble
36/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 9. Physical and chemical properties and safety characteristics
Viscosity
QuikSolution
Dynamic: 2.14 mPa·s (2.14 cP)
PfuUltra HF DNA Polymerase
Not available.
10X Reaction Buffer
Not available.
Dpn I
Not available.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Not available.
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
:
XL10-Gold 2-Mercaptoethanol
Not available.
pUC 18 DNA Control Plasmid
Not available.
Particle characteristics
Median particle size
:
QuikSolution
Not applicable.
PfuUltra HF DNA Polymerase
Not applicable.
10X Reaction Buffer
Not applicable.
Dpn I
Not applicable.
Control Primer 1 (34-mer)
Not applicable.
Control Primer 2 (34-mer)
Not applicable.
pWS4.5 Control Template
Not applicable.
QuikChange XL dNTP Mix
Not applicable.
XL10-Gold Ultracompetent cells
Not applicable.
XL10-Gold 2-Mercaptoethanol
Not applicable.
pUC 18 DNA Control Plasmid
Not applicable.
Section 10. Stability and reactivity
QuikSolution
The product is stable.
PfuUltra HF DNA Polymerase
The product is stable.
10X Reaction Buffer
The product is stable.
Dpn I
The product is stable.
Control Primer 1 (34-mer)
The product is stable.
Control Primer 2 (34-mer)
The product is stable.
pWS4.5 Control Template
The product is stable.
QuikChange XL dNTP Mix
The product is stable.
10.2 Chemical stability
:
10.1 Reactivity
:
QuikSolution
No specific test data related to reactivity available
for this product or its ingredients.
PfuUltra HF DNA Polymerase
No specific test data related to reactivity available
for this product or its ingredients.
10X Reaction Buffer
No specific test data related to reactivity available
for this product or its ingredients.
Dpn I
No specific test data related to reactivity available
for this product or its ingredients.
Control Primer 1 (34-mer)
No specific test data related to reactivity available
for this product or its ingredients.
Control Primer 2 (34-mer)
No specific test data related to reactivity available
for this product or its ingredients.
pWS4.5 Control Template
No specific test data related to reactivity available
for this product or its ingredients.
QuikChange XL dNTP Mix
No specific test data related to reactivity available
for this product or its ingredients.
XL10-Gold Ultracompetent cells
No specific test data related to reactivity available
for this product or its ingredients.
XL10-Gold 2-Mercaptoethanol
No specific test data related to reactivity available
for this product or its ingredients.
pUC 18 DNA Control Plasmid
No specific test data related to reactivity available
for this product or its ingredients.
37/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 10. Stability and reactivity
10.4 Conditions to avoid
QuikSolution
Avoid all possible sources of ignition (spark or
flame). Do not pressurize, cut, weld, braze, solder,
drill, grind or expose containers to heat or sources
of ignition. Do not allow vapor to accumulate in low
or confined areas.
PfuUltra HF DNA Polymerase
No specific data.
10X Reaction Buffer
No specific data.
Dpn I
No specific data.
Control Primer 1 (34-mer)
No specific data.
Control Primer 2 (34-mer)
No specific data.
pWS4.5 Control Template
No specific data.
QuikChange XL dNTP Mix
No specific data.
XL10-Gold Ultracompetent cells
No specific data.
XL10-Gold 2-Mercaptoethanol
No specific data.
pUC 18 DNA Control Plasmid
No specific data.
XL10-Gold Ultracompetent cells
The product is stable.
XL10-Gold 2-Mercaptoethanol
The product is stable.
pUC 18 DNA Control Plasmid
The product is stable.
QuikSolution
Reactive or incompatible with the following
materials:
oxidizing materials
PfuUltra HF DNA Polymerase
May react or be incompatible with oxidizing
materials.
10X Reaction Buffer
May react or be incompatible with oxidizing
materials.
Dpn I
May react or be incompatible with oxidizing
materials.
Control Primer 1 (34-mer)
May react or be incompatible with oxidizing
materials.
Control Primer 2 (34-mer)
May react or be incompatible with oxidizing
materials.
pWS4.5 Control Template
May react or be incompatible with oxidizing
materials.
QuikChange XL dNTP Mix
May react or be incompatible with oxidizing
:
10.5 Incompatible materials
:
10.3 Possibility of
hazardous reactions
:
QuikSolution
Under normal conditions of storage and use,
hazardous reactions will not occur.
PfuUltra HF DNA Polymerase
Under normal conditions of storage and use,
hazardous reactions will not occur.
10X Reaction Buffer
Under normal conditions of storage and use,
hazardous reactions will not occur.
Dpn I
Under normal conditions of storage and use,
hazardous reactions will not occur.
Control Primer 1 (34-mer)
Under normal conditions of storage and use,
hazardous reactions will not occur.
Control Primer 2 (34-mer)
Under normal conditions of storage and use,
hazardous reactions will not occur.
pWS4.5 Control Template
Under normal conditions of storage and use,
hazardous reactions will not occur.
QuikChange XL dNTP Mix
Under normal conditions of storage and use,
hazardous reactions will not occur.
XL10-Gold Ultracompetent cells
Under normal conditions of storage and use,
hazardous reactions will not occur.
XL10-Gold 2-Mercaptoethanol
Under normal conditions of storage and use,
hazardous reactions will not occur.
pUC 18 DNA Control Plasmid
Under normal conditions of storage and use,
hazardous reactions will not occur.
38/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 10. Stability and reactivity
10.6 Hazardous
decomposition products
QuikSolution
Under normal conditions of storage and use,
hazardous decomposition products should not be
produced.
PfuUltra HF DNA Polymerase
Under normal conditions of storage and use,
hazardous decomposition products should not be
produced.
10X Reaction Buffer
Under normal conditions of storage and use,
hazardous decomposition products should not be
produced.
Dpn I
Under normal conditions of storage and use,
hazardous decomposition products should not be
produced.
Control Primer 1 (34-mer)
Under normal conditions of storage and use,
hazardous decomposition products should not be
produced.
Control Primer 2 (34-mer)
Under normal conditions of storage and use,
hazardous decomposition products should not be
produced.
pWS4.5 Control Template
Under normal conditions of storage and use,
hazardous decomposition products should not be
produced.
QuikChange XL dNTP Mix
Under normal conditions of storage and use,
hazardous decomposition products should not be
produced.
XL10-Gold Ultracompetent cells
Under normal conditions of storage and use,
hazardous decomposition products should not be
produced.
XL10-Gold 2-Mercaptoethanol
Under normal conditions of storage and use,
hazardous decomposition products should not be
produced.
pUC 18 DNA Control Plasmid
Under normal conditions of storage and use,
hazardous decomposition products should not be
produced.
materials.
XL10-Gold Ultracompetent cells
May react or be incompatible with oxidizing
materials.
XL10-Gold 2-Mercaptoethanol
May react or be incompatible with oxidizing
materials.
pUC 18 DNA Control Plasmid
May react or be incompatible with oxidizing
materials.
:
Section 11. Toxicological information
Acute toxicity
QuikSolution
Dimethyl sulfoxide
LD50 Dermal
Rat
40000 mg/kg
-
LD50 Oral
Rat
14500 mg/kg
-
PfuUltra HF DNA
Polymerase
Glycerol
LD50 Oral
Rat
12600 mg/kg
-
Poly(oxy-1,2-ethanediyl), .
alpha.-[
(1,1,3,3-tetramethylbutyl)
phenyl]-.omega.-hydroxy-
LD50 Oral
Rat
2800 mg/kg
-
Product/ingredient name
Result
Species
Dose
Exposure
11.1 Information on toxicological effects
39/52
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 11. Toxicological information
10X Reaction Buffer
Ammonium sulphate
LD50 Oral
Rat
2840 mg/kg
-
Polyoxyethylene octyl phenyl
ether
LD50 Oral
Rat
1800 mg/kg
-
Dpn I
Glycerol
LD50 Oral
Rat
12600 mg/kg
-
XL10-Gold Ultracompetent
cells
Glycerol
LD50 Oral
Rat
12600 mg/kg
-
Dimethyl sulfoxide
LD50 Dermal
Rat
40000 mg/kg
-
LD50 Oral
Rat
14500 mg/kg
-
Potassium chloride
LD50 Oral
Rat
2600 mg/kg
-
XL10-Gold
2-Mercaptoethanol
2-Mercaptoethanol
LD50 Oral
Rat
244 mg/kg
-
Irritation/Corrosion
QuikSolution
Dimethyl sulfoxide
Eyes - Mild irritant
Rabbit
-
100 mg
-
Eyes - Mild irritant
Rabbit
-
24 hours 500
mg
-
Skin - Mild irritant
Rabbit
-
100 mg
-
Skin - Mild irritant
Rabbit
-
24 hours 500
mg
-
PfuUltra HF DNA
Polymerase
Glycerol
Eyes - Mild irritant
Rabbit
-
24 hours 500
mg
-
Skin - Mild irritant
Rabbit
-
24 hours 500
mg
-
Poly(oxy-1,2-ethanediyl), .
alpha.-[
(1,1,3,3-tetramethylbutyl)
phenyl]-.omega.-hydroxy-
Eyes - Severe irritant
Rabbit
-
1 %
-
10X Reaction Buffer
Polyoxyethylene octyl phenyl
ether
Skin - Mild irritant
Rabbit
-
24 hours 500
uL
-
Dpn I
Glycerol
Eyes - Mild irritant
Rabbit
-
24 hours 500
mg
-
Skin - Mild irritant
Rabbit
-
24 hours 500
mg
-
XL10-Gold Ultracompetent
cells
Glycerol
Eyes - Mild irritant
Rabbit
-
24 hours 500
mg
-
Skin - Mild irritant
Rabbit
-
24 hours 500
mg
-
Dimethyl sulfoxide
Eyes - Mild irritant
Rabbit
-
100 mg
-
Eyes - Mild irritant
Rabbit
-
24 hours 500
mg
-
Product/ingredient name
Result
Score
Exposure
Observation
Species
40/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 11. Toxicological information
Carcinogenicity
Conclusion/Summary
:
Not available.
Mutagenicity
Conclusion/Summary
:
Not available.
Teratogenicity
Conclusion/Summary
:
Not available.
Reproductive toxicity
Conclusion/Summary
:
Not available.
Skin - Mild irritant
Rabbit
-
100 mg
-
Skin - Mild irritant
Rabbit
-
24 hours 500
mg
-
Potassium chloride
Eyes - Mild irritant
Rabbit
-
24 hours 500
mg
-
XL10-Gold
2-Mercaptoethanol
2-Mercaptoethanol
Eyes - Severe irritant
Rabbit
-
2 mg
-
Sensitization
Not available.
Information on the likely
routes of exposure
Specific target organ toxicity (single exposure)
Specific target organ toxicity (repeated exposure)
XL10-Gold 2-Mercaptoethanol
2-Mercaptoethanol
Category 3
-
Respiratory tract
irritation
Name
Category
Name
Category
XL10-Gold 2-Mercaptoethanol
2-Mercaptoethanol
Category 2
oral
heart, liver
Aspiration hazard
Not available.
Route of
exposure
Target organs
Route of
exposure
Target organs
:
QuikSolution
Routes of entry anticipated: Oral, Dermal,
Inhalation, Eyes.
PfuUltra HF DNA Polymerase
Routes of entry anticipated: Oral, Dermal,
Inhalation, Eyes.
10X Reaction Buffer
Routes of entry anticipated: Oral, Dermal,
Inhalation, Eyes.
Dpn I
Routes of entry anticipated: Oral, Dermal,
Inhalation, Eyes.
Control Primer 1 (34-mer)
Not available.
Control Primer 2 (34-mer)
Not available.
pWS4.5 Control Template
Not available.
QuikChange XL dNTP Mix
Not available.
XL10-Gold Ultracompetent cells
Routes of entry anticipated: Oral, Dermal,
Inhalation, Eyes.
XL10-Gold 2-Mercaptoethanol
Routes of entry anticipated: Oral, Dermal,
Inhalation, Eyes.
pUC 18 DNA Control Plasmid
Not available.
Potential acute health effects
41/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 11. Toxicological information
Inhalation
:
QuikSolution
No known significant effects or critical hazards.
PfuUltra HF DNA Polymerase
No known significant effects or critical hazards.
10X Reaction Buffer
No known significant effects or critical hazards.
Dpn I
No known significant effects or critical hazards.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
No known significant effects or critical hazards.
XL10-Gold 2-Mercaptoethanol
No known significant effects or critical hazards.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
QuikSolution
No known significant effects or critical hazards.
PfuUltra HF DNA Polymerase
No known significant effects or critical hazards.
10X Reaction Buffer
No known significant effects or critical hazards.
Dpn I
No known significant effects or critical hazards.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
No known significant effects or critical hazards.
XL10-Gold 2-Mercaptoethanol
No known significant effects or critical hazards.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
:
Ingestion
Skin contact
:
QuikSolution
No known significant effects or critical hazards.
PfuUltra HF DNA Polymerase
No known significant effects or critical hazards.
10X Reaction Buffer
No known significant effects or critical hazards.
Dpn I
No known significant effects or critical hazards.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
No known significant effects or critical hazards.
XL10-Gold 2-Mercaptoethanol
May cause an allergic skin reaction.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
QuikSolution
Causes eye irritation.
PfuUltra HF DNA Polymerase
Causes eye irritation.
10X Reaction Buffer
Causes serious eye irritation.
Dpn I
Causes eye irritation.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
Causes eye irritation.
XL10-Gold 2-Mercaptoethanol
Causes serious eye damage.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
:
Eye contact
Symptoms related to the physical, chemical and toxicological characteristics
Eye contact
:
QuikSolution
Adverse symptoms may include the following:
irritation
watering
redness
PfuUltra HF DNA Polymerase
Adverse symptoms may include the following:
irritation
watering
redness
10X Reaction Buffer
Adverse symptoms may include the following:
pain or irritation
watering
redness
Dpn I
Adverse symptoms may include the following:
42/52
Date of issue :
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 11. Toxicological information
Skin contact
Ingestion
Inhalation
QuikSolution
No specific data.
PfuUltra HF DNA Polymerase
No specific data.
10X Reaction Buffer
No specific data.
Dpn I
No specific data.
Control Primer 1 (34-mer)
No specific data.
Control Primer 2 (34-mer)
No specific data.
pWS4.5 Control Template
No specific data.
QuikChange XL dNTP Mix
No specific data.
XL10-Gold Ultracompetent cells
No specific data.
XL10-Gold 2-Mercaptoethanol
Adverse symptoms may include the following:
reduced fetal weight
increase in fetal deaths
skeletal malformations
pUC 18 DNA Control Plasmid
No specific data.
QuikSolution
No specific data.
PfuUltra HF DNA Polymerase
No specific data.
10X Reaction Buffer
No specific data.
Dpn I
No specific data.
Control Primer 1 (34-mer)
No specific data.
Control Primer 2 (34-mer)
No specific data.
pWS4.5 Control Template
No specific data.
QuikChange XL dNTP Mix
No specific data.
XL10-Gold Ultracompetent cells
No specific data.
XL10-Gold 2-Mercaptoethanol
Adverse symptoms may include the following:
stomach pains
reduced fetal weight
increase in fetal deaths
QuikSolution
No specific data.
PfuUltra HF DNA Polymerase
No specific data.
10X Reaction Buffer
No specific data.
Dpn I
No specific data.
Control Primer 1 (34-mer)
No specific data.
Control Primer 2 (34-mer)
No specific data.
pWS4.5 Control Template
No specific data.
QuikChange XL dNTP Mix
No specific data.
XL10-Gold Ultracompetent cells
No specific data.
XL10-Gold 2-Mercaptoethanol
Adverse symptoms may include the following:
pain or irritation
redness
blistering may occur
reduced fetal weight
increase in fetal deaths
skeletal malformations
pUC 18 DNA Control Plasmid
No specific data.
:
:
:
irritation
watering
redness
Control Primer 1 (34-mer)
No specific data.
Control Primer 2 (34-mer)
No specific data.
pWS4.5 Control Template
No specific data.
QuikChange XL dNTP Mix
No specific data.
XL10-Gold Ultracompetent cells
Adverse symptoms may include the following:
irritation
watering
redness
XL10-Gold 2-Mercaptoethanol
Adverse symptoms may include the following:
pain
watering
redness
pUC 18 DNA Control Plasmid
No specific data.
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Section 11. Toxicological information
QuikSolution
No known significant effects or critical hazards.
PfuUltra HF DNA Polymerase
No known significant effects or critical hazards.
10X Reaction Buffer
No known significant effects or critical hazards.
Dpn I
No known significant effects or critical hazards.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
No known significant effects or critical hazards.
XL10-Gold 2-Mercaptoethanol
May cause damage to organs through prolonged or
repeated exposure. Once sensitized, a severe
allergic reaction may occur when subsequently
exposed to very low levels.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
General
:
QuikSolution
No known significant effects or critical hazards.
PfuUltra HF DNA Polymerase
No known significant effects or critical hazards.
10X Reaction Buffer
No known significant effects or critical hazards.
Dpn I
No known significant effects or critical hazards.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
No known significant effects or critical hazards.
XL10-Gold 2-Mercaptoethanol
No known significant effects or critical hazards.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
Carcinogenicity
:
QuikSolution
No known significant effects or critical hazards.
PfuUltra HF DNA Polymerase
No known significant effects or critical hazards.
10X Reaction Buffer
No known significant effects or critical hazards.
Dpn I
No known significant effects or critical hazards.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
XL10-Gold Ultracompetent cells
No known significant effects or critical hazards.
XL10-Gold 2-Mercaptoethanol
No known significant effects or critical hazards.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
Mutagenicity
:
skeletal malformations
pUC 18 DNA Control Plasmid
No specific data.
Potential chronic health effects
Delayed and immediate effects and also chronic effects from short and long term exposure
Potential immediate
effects
:
Not available.
Short term exposure
Potential delayed effects
:
Not available.
Potential immediate
effects
:
Not available.
Long term exposure
Potential delayed effects
:
Not available.
QuikSolution
No known significant effects or critical hazards.
PfuUltra HF DNA Polymerase
No known significant effects or critical hazards.
10X Reaction Buffer
No known significant effects or critical hazards.
Dpn I
No known significant effects or critical hazards.
Control Primer 1 (34-mer)
No known significant effects or critical hazards.
Control Primer 2 (34-mer)
No known significant effects or critical hazards.
pWS4.5 Control Template
No known significant effects or critical hazards.
QuikChange XL dNTP Mix
No known significant effects or critical hazards.
Reproductive toxicity
:
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Section 11. Toxicological information
Numerical measures of toxicity
Acute toxicity estimates
Product/ingredient name
QuikSolution
Dimethyl sulfoxide
14500
40000
N/A
N/A
N/A
PfuUltra HF DNA Polymerase
Glycerol
12600
N/A
N/A
N/A
N/A
Poly(oxy-1,2-ethanediyl), .alpha.-[
(1,1,3,3-tetramethylbutyl)phenyl]-.omega.-hydroxy-
500
N/A
N/A
N/A
N/A
10X Reaction Buffer
10X Reaction Buffer
98687.3
N/A
N/A
N/A
N/A
Ammonium sulphate
2840
N/A
N/A
N/A
N/A
Polyoxyethylene octyl phenyl ether
1800
N/A
N/A
N/A
N/A
Dpn I
Dpn I
130435.3
N/A
N/A
N/A
N/A
Glycerol
12600
N/A
N/A
N/A
N/A
XL10-Gold Ultracompetent cells
XL10-Gold Ultracompetent cells
136842.1
N/A
N/A
N/A
N/A
Glycerol
12600
N/A
N/A
N/A
N/A
Dimethyl sulfoxide
14500
40000
N/A
N/A
N/A
Potassium chloride
2600
N/A
N/A
N/A
N/A
XL10-Gold 2-Mercaptoethanol
XL10-Gold 2-Mercaptoethanol
4615.5
4545.5
N/A
60.7
N/A
2-Mercaptoethanol
244
200
N/A
3
N/A
Oral (mg/
kg)
Dermal
(mg/kg)
Inhalation
(gases)
(ppm)
Inhalation
(vapors)
(mg/l)
Inhalation
(dusts and
mists) (mg/
l)
XL10-Gold Ultracompetent cells
No known significant effects or critical hazards.
XL10-Gold 2-Mercaptoethanol
Suspected of damaging fertility or the unborn child.
pUC 18 DNA Control Plasmid
No known significant effects or critical hazards.
Section 12. Ecological information
12.1 Toxicity
QuikSolution
Dimethyl sulfoxide
Acute LC50 25000 ppm Fresh water
Daphnia - Daphnia magna -
Neonate
48 hours
Acute LC50 34000000 µg/l Fresh water
Fish - Pimephales promelas
96 hours
Chronic NOEC 100 ul/L Marine water
Algae - Ulva lactuca
72 hours
Chronic NOEC 100 ul/L Fresh water
Daphnia - Daphnia magna -
Juvenile (Fledgling, Hatchling,
Weanling)
21 days
PfuUltra HF DNA
Polymerase
Glycerol
Acute LC50 54000 mg/l Fresh water
Fish - Oncorhynchus mykiss
96 hours
Poly(oxy-1,2-ethanediyl), .
alpha.-[
Acute EC50 210 µg/l Fresh water
Algae - Selenastrum sp.
96 hours
Product/ingredient name
Species
Result
Exposure
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Section 12. Ecological information
(1,1,3,3-tetramethylbutyl)
phenyl]-.omega.-hydroxy-
Acute LC50 10800 µg/l Marine water
Crustaceans - Pandalus
montagui - Adult
48 hours
Acute LC50 8600 µg/l Fresh water
Daphnia - Daphnia magna -
Neonate
48 hours
Acute LC50 7200 µg/l Fresh water
Fish - Oncorhynchus mykiss
96 hours
10X Reaction Buffer
Ammonium sulphate
Chronic NOEC 7.5 mg/l Marine water
Algae - Phaeodactylum
tricornutum - Exponential growth
phase
96 hours
Polyoxyethylene octyl phenyl
ether
Acute LC50 5.85 mg/l Fresh water
Crustaceans - Ceriodaphnia
rigaudi - Neonate
48 hours
Acute LC50 11.2 mg/l Fresh water
Daphnia - Daphnia magna -
Neonate
48 hours
Acute LC50 4500 µg/l Fresh water
Fish - Pimephales promelas
96 hours
Dpn I
Glycerol
Acute LC50 54000 mg/l Fresh water
Fish - Oncorhynchus mykiss
96 hours
XL10-Gold Ultracompetent
cells
Glycerol
Acute LC50 54000 mg/l Fresh water
Fish - Oncorhynchus mykiss
96 hours
Dimethyl sulfoxide
Acute LC50 25000 ppm Fresh water
Daphnia - Daphnia magna -
Neonate
48 hours
Acute LC50 34000000 µg/l Fresh water
Fish - Pimephales promelas
96 hours
Chronic NOEC 100 ul/L Marine water
Algae - Ulva lactuca
72 hours
Chronic NOEC 100 ul/L Fresh water
Daphnia - Daphnia magna -
Juvenile (Fledgling, Hatchling,
Weanling)
21 days
Potassium chloride
Acute EC50 9.24 g/L Fresh water
Algae - Desmodesmus
subspicatus
72 hours
Acute EC50 1337000 µg/l Fresh water
Algae - Navicula seminulum
96 hours
Acute EC50 83000 µg/l Fresh water
Daphnia - Daphnia magna
48 hours
Acute LC50 9.68 mg/l Fresh water
Crustaceans - Pseudosida
ramosa - Neonate
48 hours
Acute LC50 509.65 mg/l Fresh water
Fish - Danio rerio
96 hours
12.2 Persistence and degradability
QuikSolution
Dimethyl sulfoxide
OECD 301D
Ready
Biodegradability -
Closed Bottle
Test
31 % - Not readily - 28 days
-
-
PfuUltra HF DNA
Polymerase
Glycerol
301D Ready
Biodegradability -
Closed Bottle
Test
93 % - 30 days
-
-
Dpn I
Glycerol
301D Ready
Biodegradability -
93 % - 30 days
-
-
Product/ingredient name
Test
Dose
Result
Inoculum
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Section 12. Ecological information
LogP
ow
BCF
Potential
12.3 Bioaccumulative potential
Product/ingredient name
QuikSolution
Dimethyl sulfoxide
-2.029
3.16
low
PfuUltra HF DNA
Polymerase
Glycerol
-1.76
-
low
Poly(oxy-1,2-ethanediyl), .
alpha.-[
(1,1,3,3-tetramethylbutyl)
phenyl]-.omega.-hydroxy-
2.7
78.67
low
10X Reaction Buffer
Ammonium sulphate
-5.1
-
low
Polyoxyethylene octyl phenyl
4.86
-
high
Product/ingredient name
Aquatic half-life
Photolysis
Biodegradability
QuikSolution
Dimethyl sulfoxide
-
-
Not readily
10X Reaction Buffer
Ammonium sulphate
-
-
Readily
Polyoxyethylene octyl phenyl
ether
-
-
Readily
XL10-Gold Ultracompetent
cells
Dimethyl sulfoxide
-
-
Not readily
Potassium chloride
-
-
Readily
XL10-Gold
2-Mercaptoethanol
2-Mercaptoethanol
-
-
Not readily
Closed Bottle
Test
XL10-Gold Ultracompetent
cells
Glycerol
301D Ready
Biodegradability -
Closed Bottle
Test
93 % - 30 days
-
-
Dimethyl sulfoxide
OECD 301D
Ready
Biodegradability -
Closed Bottle
Test
31 % - Not readily - 28 days
-
-
XL10-Gold
2-Mercaptoethanol
2-Mercaptoethanol
OECD 310
Ready
Biodegradability -
CO₂
in Sealed
Vessels
(Headspace Test)
69 % - Not readily - 60 days
20 mg/l
-
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Section 12. Ecological information
12.5 Other adverse effects
:
No known significant effects or critical hazards.
ether
Dpn I
Glycerol
-1.76
-
low
XL10-Gold Ultracompetent
cells
Glycerol
-1.76
-
low
Dimethyl sulfoxide
-1.35
3.16
low
Potassium chloride
-0.46
-
low
XL10-Gold
2-Mercaptoethanol
2-Mercaptoethanol
-0.056
-
low
Soil/water partition
coefficient (K
OC
)
:
Not available.
12.4 Mobility in soil
Section 13. Disposal considerations
The generation of waste should be avoided or minimized wherever possible. Disposal of
this product, solutions and any by-products should at all times comply with the
requirements of environmental protection and waste disposal legislation and any regional
local authority requirements. Dispose of surplus and non-recyclable products via a
licensed waste disposal contractor. Waste should not be disposed of untreated to the
sewer unless fully compliant with the requirements of all authorities with jurisdiction.
Waste packaging should be recycled. Incineration or landfill should only be considered
when recycling is not feasible. This material and its container must be disposed of in a
safe way. Care should be taken when handling emptied containers that have not been
cleaned or rinsed out. Empty containers or liners may retain some product residues.
Avoid dispersal of spilled material and runoff and contact with soil, waterways, drains
and sewers.
:
Disposal methods
Disposal should be in accordance with applicable regional, national and local laws and regulations. Local
regulations may be more stringent than regional or national requirements.
The information presented below only applies to the material as supplied. The identification based on
characteristic(s) or listing may not apply if the material has been used or otherwise contaminated. It is the
responsibility of the waste generator to determine the toxicity and physical properties of the material generated to
determine the proper waste identification and disposal methods in compliance with applicable regulations.
Refer to Section 7: HANDLING AND STORAGE and Section 8: EXPOSURE CONTROLS/PERSONAL PROTECTION
for additional handling information and protection of employees.
13.1 Waste treatment methods
Section 14. Transport information
DOT / TDG / Mexico / IMDG /
IATA
:
Not regulated.
Special precautions for user
Transport within user’s premises:
always transport in closed containers that are
upright and secure. Ensure that persons transporting the product know what to do in the
event of an accident or spillage.
:
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 14. Transport information
Transport in bulk according
to IMO instruments
:
Not available.
Section 15. Regulatory information
U.S. Federal regulations
Clean Water Act (CWA) 311
: Edetic acid
:
Clean Air Act Section 112
(b) Hazardous Air
Pollutants (HAPs)
:
Listed
Clean Air Act Section 602
Class I Substances
:
Not listed
Clean Air Act Section 602
Class II Substances
:
Not listed
DEA List I Chemicals
(Precursor Chemicals)
:
Not listed
DEA List II Chemicals
(Essential Chemicals)
:
Not listed
TSCA 8(a) PAIR
: Polyoxyethylene octyl phenyl ether; Poly(oxy-1,2-ethanediyl), .alpha.-[
(1,1,3,3-tetramethylbutyl)phenyl]-.omega.-hydroxy-
TSCA 8(a) CDR Exempt/Partial exemption
: Not determined
SARA 302/304
SARA 304 RQ
:
Not applicable.
No products were found.
Composition/information on ingredients
SARA 311/312
Classification
:
QuikSolution
FLAMMABLE LIQUIDS - Category 4
EYE IRRITATION - Category 2B
PfuUltra HF DNA Polymerase
EYE IRRITATION - Category 2B
10X Reaction Buffer
EYE IRRITATION - Category 2A
Dpn I
EYE IRRITATION - Category 2B
Control Primer 1 (34-mer)
Not applicable.
Control Primer 2 (34-mer)
Not applicable.
pWS4.5 Control Template
Not applicable.
QuikChange XL dNTP Mix
Not applicable.
XL10-Gold Ultracompetent cells
EYE IRRITATION - Category 2B
XL10-Gold 2-Mercaptoethanol
SERIOUS EYE DAMAGE - Category 1
SKIN SENSITIZATION - Category 1
TOXIC TO REPRODUCTION - Category 2
pUC 18 DNA Control Plasmid
Not applicable.
15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture
QuikSolution
Dimethyl sulfoxide
100
FLAMMABLE LIQUIDS - Category 4
EYE IRRITATION - Category 2B
PfuUltra HF DNA Polymerase
Glycerol
≥50 - ≤75
EYE IRRITATION - Category 2B
10X Reaction Buffer
Ammonium sulphate
≤3
EYE IRRITATION - Category 2A
Polyoxyethylene octyl phenyl
ether
<2.5
ACUTE TOXICITY (oral) - Category 4
SKIN IRRITATION - Category 2
SERIOUS EYE DAMAGE - Category 1
Dpn I
Name
%
Classification
Composition/information on ingredients
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Section 15. Regulatory information
The following components are listed: GLYCERINE MIST
Massachusetts
:
SARA 313
Product name
CAS number
%
SARA 313 notifications must not be detached from the SDS and any copying and redistribution of the SDS shall include
copying and redistribution of the notice attached to copies of the SDS subsequently redistributed.
10X Reaction Buffer
Ammonium sulphate
7783-20-2
≤3
10X Reaction Buffer
Ammonium sulphate
7783-20-2
≤3
Form R - Reporting
requirements
Supplier notification
California Prop. 65
New York
:
None of the components are listed.
New Jersey
:
The following components are listed: GLYCERIN; DIMETHYL SULFOXIDE
Pennsylvania
:
The following components are listed: 1,2,3-PROPANETRIOL
State regulations
International regulations
Chemical Weapon Convention List Schedules I, II & III Chemicals
Montreal Protocol
Not listed.
Stockholm Convention on Persistent Organic Pollutants
Not listed.
Rotterdam Convention on Prior Informed Consent (PIC)
Not listed.
UNECE Aarhus Protocol on POPs and Heavy Metals
Not listed.
Inventory list
Australia
:
Not determined.
Canada
:
All components are listed or exempted.
This product does not require a Safe Harbor warning under California Prop. 65.
Glycerol
≥50 - ≤75
EYE IRRITATION - Category 2B
XL10-Gold Ultracompetent
cells
Glycerol
≥10 - ≤25
EYE IRRITATION - Category 2B
Dimethyl sulfoxide
≤10
FLAMMABLE LIQUIDS - Category 4
EYE IRRITATION - Category 2B
Sucrose
≤10
COMBUSTIBLE DUSTS
Potassium chloride
≤3
EYE IRRITATION - Category 2B
XL10-Gold 2-Mercaptoethanol
2-Mercaptoethanol
≤5
FLAMMABLE LIQUIDS - Category 4
ACUTE TOXICITY (oral) - Category 3
ACUTE TOXICITY (dermal) - Category 2
ACUTE TOXICITY (inhalation) - Category 3
SKIN IRRITATION - Category 2
SERIOUS EYE DAMAGE - Category 1
SKIN SENSITIZATION - Category 1A
TOXIC TO REPRODUCTION - Category 2
SPECIFIC TARGET ORGAN TOXICITY (SINGLE EXPOSURE) (Respiratory tract
irritation) - Category 3
SPECIFIC TARGET ORGAN TOXICITY (REPEATED EXPOSURE) - Category 2
Not listed.
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 15. Regulatory information
China
:
Not determined.
Japan
:
Japan inventory (CSCL)
: Not determined.
Japan inventory (ISHL)
: Not determined.
Republic of Korea
:
Not determined.
New Zealand
:
Not determined.
Philippines
:
Not determined.
Taiwan
:
All components are listed or exempted.
Turkey
:
Not determined.
United States
:
All components are active or exempted.
Thailand
:
Not determined.
Viet Nam
:
Not determined.
Eurasian Economic Union
:
Russian Federation inventory
: All components are listed or exempted.
Section 16. Other information
History
Date of issue
Version
Date of previous issue
:
:
:
Key to abbreviations
:
12/16/2022
05/26/2022
8.1
Procedure used to derive the classification
Classification
Justification
QuikSolution
FLAMMABLE LIQUIDS - Category 4
On basis of test data
EYE IRRITATION - Category 2B
On basis of test data
PfuUltra HF DNA Polymerase
EYE IRRITATION - Category 2B
Calculation method
10X Reaction Buffer
EYE IRRITATION - Category 2A
Calculation method
AQUATIC HAZARD (LONG-TERM) - Category 3
Calculation method
Dpn I
EYE IRRITATION - Category 2B
Calculation method
XL10-Gold Ultracompetent cells
EYE IRRITATION - Category 2B
Calculation method
XL10-Gold 2-Mercaptoethanol
SERIOUS EYE DAMAGE - Category 1
Calculation method
SKIN SENSITIZATION - Category 1
Calculation method
TOXIC TO REPRODUCTION - Category 2
Calculation method
SPECIFIC TARGET ORGAN TOXICITY (REPEATED EXPOSURE) - Category 2
Calculation method
AQUATIC HAZARD (LONG-TERM) - Category 3
Calculation method
ATE = Acute Toxicity Estimate
BCF = Bioconcentration Factor
GHS = Globally Harmonized System of Classification and Labelling of Chemicals
IATA = International Air Transport Association
IBC = Intermediate Bulk Container
IMDG = International Maritime Dangerous Goods
LogPow = logarithm of the octanol/water partition coefficient
MARPOL = International Convention for the Prevention of Pollution From Ships, 1973
as modified by the Protocol of 1978. ("Marpol" = marine pollution)
N/A = Not available
51/52
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QuikChange II XL Site-Directed Mutagenesis Kit, Part Number 200522
Section 16. Other information
Indicates information that has changed from previously issued version.
Notice to reader
Disclaimer: The information contained in this document is based on Agilent’s state of knowledge at the time of
preparation. No warranty as to its accurateness, completeness or suitability for a particular purpose is expressed
or implied.
UN = United Nations
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